Chondrocytes

1. Front Matter

   Pages i-xiii

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2. Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D

   • Rodrigo A. Somoza, Jean F. Welter

   Pages 1-12

3. Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage

   • Ilona Uzieliene, Jaroslav Denkovskij, Eiva Bernotiene, Ursule Kalvaityte, Raminta Vaiciuleviciute, Yolande F. M. Ramos et al.

   Pages 13-22

4. Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse

   • Ioannis Kanakis, Mohammad Alhashmi, Ke Liu, Craig Keenan, Lorenzo Ramos Mucci, Blandine Poulet et al.

   Pages 23-38

5. Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures

   • Abdul Haseeb, Véronique Lefebvre

   Pages 39-51

6. Fetal Growth Plate Cartilage : Histological and Immunohistochemical Techniques

   • Zachary Tata, Christophe Merceron, Ernestina Schipani

   Pages 53-84

7. Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization

   • Charles de Charleroy, Abdul Haseeb, Véronique Lefebvre

   Pages 85-92

8. MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Joints

   • Helal Endisha, Mohit Kapoor

   Pages 93-103

9. Laser Capture Microdissection of Mouse Growth Plate Cartilage

   • Bijal Kikani, Julian C. Lui

   Pages 105-119

10. RNA Isolation from Articular Cartilage Tissue

    • Hongjun Zheng, Audrey McAlinden

    Pages 121-133

11. Small Nucleolar RNA Expression Profiling in Cartilage

    • Mandy J. Peffers, Andy Cremers, Tim J. M. Welting

    Pages 135-149

12. MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes

    • Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Yoshiaki Ito, Ryo Nakamichi, Martin Lotz et al.

    Pages 151-166

13. ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes

    • Akira Yamakawa, Hironori Hojo, Shinsuke Ohba

    Pages 167-178

14. MicroRNAs and Regulation of Autophagy in Chondrocytes

    • Yolande F. M. Ramos, Ali Mobasheri

    Pages 179-194

15. Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes

    • Yun Wang, Liang-Yu Chen, Ru Liu-Bryan

    Pages 195-213

16. Assessing Chondrocyte Status by Immunofluorescence-Mediated Localization of Parkin Relative to Mitochondria

    • Mohammad Y. Ansari, Tariq M. Haqqi

    Pages 215-224

17. Back Matter

    Pages 225-229

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This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, in vivo assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained in vivo, just isolated from their tissue (ex vivo), or cultured in vitro. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Chondrocytes: Methods andProtocols is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.

• Cartilage biology
• Extracellular matrix
• Collagen
• Skeletal diseases
• Pathology
• Experimental models
• Degenerative disorders

• Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, USA

  Tariq M. Haqqi

• Department of Surgery, The Children's Hospital of Philadelphia, Philadelphia, USA

  Véronique Lefebvre

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