Overview
- Editors:
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Edward M. Fox
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Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK
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Hélène Bierne
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INRAE, AgroParisTech, Micalis Institute,, Université Paris-Saclay, Jouy-en-Josas, France
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Beatrix Stessl
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Institute of Food Safety Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (19 protocols)
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Detection, Quantification, and Confirmation
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- Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, Paula Teixeira
Pages 3-16
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- Snehal R. Jadhav, Rohan M. Shah, Enzo A. Palombo
Pages 17-29
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- Patrick Mester, Anna Kristina Witte, Peter Rossmanith
Pages 31-40
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- Anna Kristina Witte, Patrick Mester, Peter Rossmanith
Pages 41-53
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Subtyping Approaches
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- Karen Hunt, Kieran Jordan
Pages 79-88
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- Beatrix Stessl, Martin Wagner, Werner Ruppitsch
Pages 89-103
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Genotypic and Phenotypic Characterization
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Front Matter
Pages 105-105
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- Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, Edward M. Fox
Pages 107-113
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- Jessica A. Gray, P. Scott Chandry, John P. Bowman, Edward M. Fox
Pages 115-122
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- Maud Darsonval, Marina Grégoire, Julien Deschamps, Romain Briandet
Pages 123-136
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- Tiago Santos, Michel Hébraud
Pages 137-153
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Strain Manipulation
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Front Matter
Pages 155-155
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- Aidan Casey, Olivia McAuliffe
Pages 157-163
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- Kathrin Rychli, Eva Wagner, Caitriona M. Guinane, Karen Daly, Colin Hill, Paul D. Cotter
Pages 165-175
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- Cameron Parsons, Reha Azizoglu, Driss Elhanafi, Sophia Kathariou
Pages 177-185
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Host-Pathogen Interactions
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Front Matter
Pages 187-187
About this book
This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.
Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.
Editors and Affiliations
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Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK
Edward M. Fox
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INRAE, AgroParisTech, Micalis Institute,, Université Paris-Saclay, Jouy-en-Josas, France
Hélène Bierne
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Institute of Food Safety Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria
Beatrix Stessl