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Mitochondrial Gene Expression

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2192)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. In Vitro Analysis of mtDNA Replication

    • Jay P. Uhler, Maria Falkenberg
    Pages 1-20
  3. In Vivo Analysis of mtDNA Replication at the Single Molecule Level and with High Resolution

    • Marco Tigano, Aaron Fraser Phillips, Agnel Sfeir
    Pages 21-34
  4. In Vitro Reconstitution of Human Mitochondrial Transcription

    • Azadeh Sarfallah, Dmitry Temiakov
    Pages 35-41
  5. Investigating Mitochondrial Transcriptomes and RNA Processing Using Circular RNA Sequencing

    • Irina Kuznetsova, Oliver Rackham, Aleksandra Filipovska
    Pages 43-57
  6. Detection of 5-formylcytosine in Mitochondrial Transcriptome

    • Lindsey Van Haute, Michal Minczuk
    Pages 59-68
  7. Quantitative Proteomics in Drosophila with Holidic Stable-Isotope Labeling of Amino Acids in Fruit Flies (SILAF)

    • Florian A. Schober, Ilian Atanassov, Christoph Freyer, Anna Wredenberg
    Pages 75-87Open Access
  8. Mass Spectrometric Analysis of Mitochondrial RNA Modifications

    • Yuma Ishigami, Tsutomu Suzuki, Takeo Suzuki
    Pages 89-101
  9. High-Throughput Detection of mtDNA Mutations Leading to tRNA Processing Errors

    • Marita Annika Isokallio, James Bruce Stewart
    Pages 117-132
  10. High-Throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells

    • Anna V. Kotrys, Lukasz S. Borowski, Roman J. Szczesny
    Pages 133-146
  11. RNA Crosslinking to Analyze the Mitochondrial RNA-Binding Proteome

    • Selma L. van Esveld, Johannes N. Spelbrink
    Pages 147-158
  12. Visualizing Mitochondrial Ribosomal RNA and Mitochondrial Protein Synthesis in Human Cell Lines

    • Matthew Zorkau, Yasmin Proctor-Kent, Rolando Berlinguer-Palmini, Andrew Hamilton, Zofia M. Chrzanowska-Lightowlers, Robert N. Lightowlers
    Pages 159-181
  13. Mitoribosome Profiling from Human Cell Culture: A High Resolution View of Mitochondrial Translation

    • Sarah F. Pearce, Miriam Cipullo, Betty Chung, Ian Brierley, Joanna Rorbach
    Pages 183-196
  14. Application of Cryo-EM for Visualization of Mitoribosomes

    • Vivek Singh, Alexey Amunts
    Pages 197-210
  15. Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes

    • Austin Choi, Antoni Barrientos
    Pages 211-226
  16. The Analysis of Yeast Mitochondrial Translation

    • Andreas Carlström, Magdalena Rzepka, Martin Ott
    Pages 227-242
  17. In Situ Studies of Mitochondrial Translation by Cryo-Electron Tomography

    • Robert Englmeier, Friedrich Förster
    Pages 243-268
  18. Mitochondrial Complexome Profiling

    • Heiko Giese, Jana Meisterknecht, Juliana Heidler, Ilka Wittig
    Pages 269-285

About this book

This volume details the most recent advancements in the field of mitochondrial gene expression. Chapters guide readers through methods and protocols on mtDNA replication, transcription, and translation to membrane insertion of the mtDNA-encoded protein products.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mitochondrial Gene Expression: Methods and Protocols aims to provide complementary approaches and practical guidelines.

 Chapter 7 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK

    Michal Minczuk

  • Division of Molecular Metabolism, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Solna, Sweden, Institutet Laboratory, Max Planck Institute Biology of Ageing - Karolinska, Karolinska Institutet, Stockholm, Sweden

    Joanna Rorbach

Bibliographic Information

  • Book Title: Mitochondrial Gene Expression

  • Book Subtitle: Methods and Protocols

  • Editors: Michal Minczuk, Joanna Rorbach

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0834-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0833-3Published: 24 November 2020

  • Softcover ISBN: 978-1-0716-0836-4Published: 24 November 2020

  • eBook ISBN: 978-1-0716-0834-0Published: 23 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 333

  • Number of Illustrations: 19 b/w illustrations, 40 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access