Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2187)
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Table of contents (20 protocols)
Keywords
About this book
This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning moreabout the function of lipid rafts in many areas of cell biology and medicine.
Editors and Affiliations
Bibliographic Information
Book Title: Lipid Rafts
Book Subtitle: Methods and Protocols
Editors: Erhard Bieberich
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0814-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-0813-5Published: 09 August 2020
Softcover ISBN: 978-1-0716-0816-6Published: 10 August 2021
eBook ISBN: 978-1-0716-0814-2Published: 08 August 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 350
Number of Illustrations: 17 b/w illustrations, 62 illustrations in colour
Topics: Lipidology, Mass Spectrometry