Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2176)
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Table of contents (17 protocols)
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Front Matter
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Basics and Introduction
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Front Matter
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Design of Gapmers and Strategies
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Front Matter
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In Vitro/In Vivo Evaluation of Gapmers
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Front Matter
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About this book
This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated RNA knockdown for basic research and therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Gapmers: Methods and Protocols aims to provide diverse applications of gapmers along with protocols that will assist readers in moving the frontier.
Editors and Affiliations
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Department of Medical Genetics, University of Alberta, Edmonton, Canada
Toshifumi Yokota, Rika Maruyama
Bibliographic Information
Book Title: Gapmers
Book Subtitle: Methods and Protocols
Editors: Toshifumi Yokota, Rika Maruyama
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0771-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0770-1Published: 01 September 2020
Softcover ISBN: 978-1-0716-0773-2Published: 01 September 2021
eBook ISBN: 978-1-0716-0771-8Published: 31 August 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 247
Number of Illustrations: 10 b/w illustrations, 29 illustrations in colour
Topics: Human Genetics