RNA Interference and Cancer Therapy

1. Front Matter

   Pages i-xiii

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2. siRNA Library Screening to Identify Complementary Therapeutic Pairs in Triple-Negative Breast Cancer Cells

   • Bindu Thapa, KC Remant, Hasan Uludağ

   Pages 1-19

3. In Vitro Evaluation of Candidate Gene Targets for Cancer Therapy

   • Xing Fei Tan, Wei Xuan Teo, George W. Yip

   Pages 21-30

4. siRNA-Based Drug Targeting Human Bcl-xL Against Cancers

   • Yoshifumi Takei

   Pages 31-40

5. Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly Active siRNAs

   • Phillip A. Angart, Kwasi Adu-Berchie, Rebecca J. Carlson, Daniel B. Vocelle, Christina Chan, S. Patrick Walton

   Pages 41-56

6. VEGFA Gene Silencing in CXCR4-Expressing Cells via siRNA Delivery by Means of Targeted Peptide Carrier

   • Anna A. Egorova, Marianna A. Maretina, Anton V. Kiselev

   Pages 57-68

7. Design Strategy to Access siRNA-Encapsulating DNA “Nanosuitcases” That Can Conditionally Release Their Cargo

   • Katherine E. Bujold, Hassan H. Fakih, Hanadi F. Sleiman

   Pages 69-81

8. Combinatorial siRNA Polyplexes for Receptor Targeting

   • Dian-Jang Lee, Ernst Wagner

   Pages 83-98

9. Synthesis of Doxorubicin and miRNA Stimuli-Sensitive Conjugates for Combination Therapy

   • Daniel F. Costa, Can Sarisozen, Vladimir P. Torchilin

   Pages 99-109

10. Minicell-Based Targeted Delivery of shRNA to Cancer Cells: An Experimental Protocol

    • Mehul Jivrajani, Manish Nivsarkar

    Pages 111-139

11. Targeted Delivery of miRNA Antagonists to Myeloid Cells In Vitro and In Vivo

    • Yu-Lin Su, Piotr Swiderski, Guido Marcucci, Marcin Kortylewski

    Pages 141-150

12. Systemic Modulation of Lymphocyte Subsets Using siRNAs Delivered via Targeted Lipid Nanoparticles

    • Inbal Hazan-Halevy, Daniel Rosenblum, Srinivas Ramishetti, Dan Peer

    Pages 151-159

13. Targeting Cancer with Peptide RNAi Nanoplexes

    • A. James Mixson, Qixin Leng, Szu-Ting Chou, Martin C. Woodle

    Pages 161-180

14. Preparation of a Carrier to Achieve In Vivo Delivery of siRNA: The Example of Chitosan-Coated Poly(isobutylcyanoacrylate) Nanoparticles

    • Christine Vauthier

    Pages 181-194

15. Monoclonal Antibody-Conjugated Dendritic Nanostructures for siRNA Delivery

    • Hitesh Kulhari, Ashok K. Jangid, David J. Adams

    Pages 195-201

16. Synthesis of Gold Nanoparticles for Gene Silencing

    • Claudia Tortiglione, Jesús M de la Fuente

    Pages 203-214

17. Design of Eco-Friendly Gold Nanoparticles for Cancer Treatment

    • Deep Pooja, Ramakrishna Sistla

    Pages 215-221

18. 64Cu-Labeled Aptamers for Tumor-Targeted Radionuclide Delivery

    • Lei Kang, Zachary T. Rosenkrans, Weibo Cai

    Pages 223-231

19. Silencing PRDM14 via Oligonucleotide Therapeutics Suppresses Tumorigenicity and Metastasis of Breast Cancer

    • Hiroaki Taniguchi, Kohzoh Imai

    Pages 233-243

20. Biodrug Suppresses Breast and Colorectal Cancer in Murine Models

    • Syed Sultan Beevi, Naveen Kumar Tangudu, Vinod Kumar Verma, Lekha Dinesh Kumar

    Pages 245-263

This comprehensive volume collects a repertoire of techniques for the adoption and exploitation of RNA interference (RNAi) as a fertile strategy to develop the bio-drugs of future in the vital field of cancer research. Exploring the immense ‘bench to bedside’ potential of RNAi as a therapeutic molecule, the book focuses many of its chapters on nanoparticle-based targeted deliveries, including aptamers, as well as techniques for using RNAi technology to combat various cancers in preclinical studies. Each of the 26 chapters is authored by the pioneers in the respective areas and thus provides a practical and empirical account of the techniques that could be used as benchmark tools in the area of cancer research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, RNA Interference and Cancer Therapy: Methods and Protocols provides the necessary guidance for both novice and professional researchers who intend to discover and innovate newer means of using RNAi technology to combat cancer, one of the greatest scourges of human health.

• Cancer treatment
• Therapeutic tools
• RNAi-based strategies
• Bio-drugs
• Drug development
• Targeted delivery
• Nanoparticles

• Cancer Biology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India

  Lekha Dinesh Kumar

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