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Autophagy in Differentiation and Tissue Maintenance

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

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  • Includes cutting-edge methods and protocols for the study of autophagy
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1854)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Visualization and Measurement of Multiple Components of the Autophagy Flux

    • Tracey Evans, Robert Button, Oleg Anichtchik, Shouqing Luo
    Pages 1-12

  3. Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells

    • Kiran Suhas Nilangekar, Bhupendra V. Shravage
    Pages 13-20

  4. Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells

    • Rohan S. Kulkarni, Manmohan S. Bajaj, Vaijayanti P. Kale
    Pages 21-34

  5. Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement

    • Kristyn Gumpper, Matthew Sermersheim, Michael X. Zhu, Pei-Hui Lin
    Pages 35-43

  6. Autophagy in Adipocyte Differentiation

    • Zhipeng Tao, Longhua Liu, Louise D. Zheng, Zhiyong Cheng
    Pages 45-53

  7. Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

    • Sinem Tunçer, Sreeparna Banerjee
    Pages 55-70

  8. Assessing Autophagy in the Leydig Cells

    • Hui Gao, Chao Liu, Wei Li
    Pages 71-85

  9. Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells

    • Javad Alizadeh, Shahla Shojaei, Adel Sepanjnia, Mohammad Hashemi, Eftekhar Eftekharpour, Saeid Ghavami
    Pages 87-103

  10. Autophagy in Zebrafish Extraocular Muscle Regeneration

    • Alfonso Saera-Vila, Phillip E. Kish, Alon Kahana
    Pages 105-117

  11. The Detection Techniques for Autophagy-Associated Cell Death-Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry

    • Melek Ozturk, Ozlem Dagdeviren Ozsoylemez, Fatma Kaya Dagistanli
    Pages 119-130

  12. Cloning of Autophagy-Related MicroRNAs

    • Deniz Gulfem Ozturk, Muhammed Kocak, Devrim Gozuacik
    Pages 131-146

  13. Assays to Monitor Aggrephagy in Drosophila Brain

    • Anne-Claire Jacomin, Ioannis P. Nezis
    Pages 147-157

  14. Methods for Monitoring Autophagy in Silkworm Organs

    • Aurora Montali, Morena Casartelli, Daniele Bruno, Annalisa Grimaldi, Gianluca Tettamanti
    Pages 159-174

  15. Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, and ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia

    • Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin, Yongquan Hu, Tian Meng et al.
    Pages 175-185

  16. Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening

    • Georgios Konstantinidis, Sonja Sievers, Yao-Wen Wu
    Pages 187-195

  17. Porcine Cell-Free System to Study Mammalian Sperm Mitophagy

    • Won-Hee Song, Peter Sutovsky
    Pages 197-207

  18. Monitoring and Measuring Mammalian Autophagy

    • Sujit K. Bhutia, Prakash P. Praharaj, Chandra S. Bhol, Debasna P. Panigrahi, Kewal K. Mahapatra, Srimanta Patra et al.
    Pages 209-222

  19. Back Matter

    Pages 223-228
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Keywords

About this book

This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Autophagy in Differentiation and Tissue Maintenance

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8748-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8747-4Published: 01 September 2018

  • Softcover ISBN: 978-1-4939-9382-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8748-1Published: 31 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 228

  • Number of Illustrations: 2 b/w illustrations, 38 illustrations in colour

  • Topics: Cell Biology

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