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Galectins

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Sean R. Stowell

    1. Joint Program in Transfusion Medicine Brigham and Women’s Hospital, Harvard Medical School, Boston, USA

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  2. Connie M. Arthur

    1. Joint Program in Transfusion Medicine Brigham and Women’s Hospital, Harvard Medical School, Boston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Richard D. Cummings

    1. Department of Surgery Beth Israel Deaconess Medical Center National Center for Functional Glycomics, Harvard Medical School, Boston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2442)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions

    • Hans Verkerke, Marcelo Dias-Baruffi, Richard D. Cummings, Connie M. Arthur, Sean R. Stowell
    Pages 1-40

  3. Cloning, Expression, and Purification of Galectins for In Vitro Studies

    • Paul A. Poland, Carol L. Kinlough, Rebecca P. Hughey
    Pages 41-54

  4. Purification of Recombinant Galectins from Different Species Using Distinct Affinity Chromatography Methods

    • Anu Paul, Shang-Chuen Wu, Kashyap R. Patel, Alex D. Ho, Jerry William Lynn Allen, Hans Verkerke et al.
    Pages 55-74

  5. Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation

    • Shang-Chuen Wu, Anu Paul, Richard D. Cummings, Christa L. Feasley, Connie M. Arthur, Sean R. Stowell
    Pages 75-87

  6. Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method

    • Priyanka D. Kadav, Jared L. Edwards, Jessica Krycia, Purnima Bandyopadhyay, Tarun K. Dam
    Pages 89-103

  7. Introducing 77Se NMR Spectroscopy to Analyzing Galectin –Ligand Interaction

    • Mária Raics, István Timári, László Szilágyi, Hans-Joachim Gabius, Katalin E. Kövér
    Pages 105-123

  8. Evaluation of Galectin Binding by Surface Plasmon Resonance

    • Padmaja Mehta-D’souza
    Pages 125-135

  9. Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein

    • Jared L. Edwards, Priyanka D. Kadav, Purnima Bandyopadhyay, Tarun K. Dam
    Pages 137-150

  10. Examining Galectin Binding Specificity Using Glycan Microarrays

    • Sean R. Stowell, Lilian C. Rodrigues, Marcelo Dias-Baruffi, Richard D. Cummings, Connie M. Arthur
    Pages 151-168

  11. Mechanism of Mucin Recognition by Lectins : A Thermodynamic Study

    • Tarun K. Dam, Jared L. Edwards, Priyanka D. Kadav, C. Fred Brewer
    Pages 169-185

  12. Examination of Whole-Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis

    • Anne Leppänen, Connie M. Arthur, Sean R. Stowell, Richard D. Cummings
    Pages 187-203

  13. Dissecting Context-Specific Galectin Binding Using Glycoengineered Cell Libraries

    • Mathias Ingemann Nielsen, Hans H. Wandall
    Pages 205-214

  14. Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins

    • Kashyap R. Patel, Adam W. Barb, Sean R. Stowell
    Pages 215-232

  15. Transformation of Agrocybe cylindracea Galectin into αGalNAc-Specific Lectin

    • Dan Hu, Jun Hirabayashi
    Pages 233-245

  16. What Happens If a Human Galectin Enters the Endoplasmic Reticulum?

    • Tanja J. Kutzner, Alonso M. Higuero, Martina Süßmair, Michael Hingar, Herbert Kaltner, Ingo Lindner et al.
    Pages 247-288

  17. Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Miccroscopy

    • Daniel Giuliano Cerri, Lilian Cataldi Rodrigues, Marise Lopes Fermino, Marcelo Papoti, Richard D. Cummings, Sean R. Stowell et al.
    Pages 289-306

  18. Exploring the Galectin Network by Light and Fluorescence Microscopy

    • Gabriel García Caballero, Joachim C. Manning, Adele Gabba, Donella Beckwith, Forrest G. FitzGerald, Tanja J. Kutzner et al.
    Pages 307-338

  19. Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners

    • Thais Canassa De Leo, Sofia Nascimento dos Santos, Emerson Soares Bernardes, Richard D. Cummings, Sean R. Stowell, Marcelo Dias-Baruffi
    Pages 339-352

  20. Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles

    • Ming-Hsiang Hong, I-Chun Weng, Fang-Yen Li, Fu-Tong Liu
    Pages 353-365
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Keywords

About this book

This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field. 

 


Editors and Affiliations

  • Joint Program in Transfusion Medicine Brigham and Women’s Hospital, Harvard Medical School, Boston, USA

    Sean R. Stowell, Connie M. Arthur

  • Department of Surgery Beth Israel Deaconess Medical Center National Center for Functional Glycomics, Harvard Medical School, Boston, USA

    Richard D. Cummings

Bibliographic Information

  • Book Title: Galectins

  • Book Subtitle: Methods and Protocols

  • Editors: Sean R. Stowell, Connie M. Arthur, Richard D. Cummings

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2055-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2054-0Published: 24 March 2022

  • Softcover ISBN: 978-1-0716-2057-1Published: 25 March 2023

  • eBook ISBN: 978-1-0716-2055-7Published: 23 March 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIX, 730

  • Number of Illustrations: 35 b/w illustrations, 86 illustrations in colour

  • Topics: Protein Science

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