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Cancer Immunoprevention

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Florencia McAllister

    1. The University of Texas MD Anderson Cancer Center, Houston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2435)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Mechanisms of Antitumor Immunity and Immunosurveillance

    • Chirayu Mohindroo, Nese Unver
    Pages 1-6

  3. Targets and Strategies for Cancer Immunoprevention

    • Nese Unver, Chirayu Mohindroo
    Pages 7-17

  4. Discovery of Targets for Cancer Immunoprevention

    • Paola Cappello, Sara Bulfamante, Giorgia Mandili, Francesco Novelli
    Pages 19-33

  5. Exosome-related Methods and Potential Use as Vaccines

    • Pinar Kanlikilicer
    Pages 35-41

  6. Isolation and Maintenance of Tumor-Infiltrating Lymphocytes for Translational and Clinical Applications: Established Methods and New Developments

    • Orenthial J. Fulbright, Marie-Andrée Forget, Cara Haymaker, Chantale Bernatchez
    Pages 43-71

  7. Monoclonal Antibodies Generation: Updates and Protocols on Hybridoma Technology

    • Ahmed Muhsin, Roberto Rangel, Long Vien, Laura Bover
    Pages 73-93

  8. Transcriptomic-Assisted Immune and Neoantigen Profiling in Premalignancy

    • Kyle Chang, Florencia McAllister, Eduardo Vilar
    Pages 95-105

  9. Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence

    • Pompeyo R. Quesada, Erick Riquelme, Jared Burks, Amanda Rakoski, Ismet Sahin, Florencia McAllister
    Pages 107-127

  10. Cytometry of Mass for Murine Immunoprofiling

    • Vidhi Chandra, Florencia McAllister, Jared K. Burks
    Pages 129-137

  11. Interrogating the Tumor Microenvironment by Deep Tissue Immunoprofiling

    • Nicolas Llosa
    Pages 139-156

  12. Proteomic Profiling of the Tumor Microenvironment

    • Michela Capello, Hiroyuki Katayama, Samir M. Hanash
    Pages 157-167

  13. Metabolic Imaging Using Hyperpolarization for Assessment of Premalignancy

    • Shivanand Pudakalakatti, Priyank Raj, Travis C. Salzillo, José S. Enriquez, Dontrey Bourgeois, Prasanta Dutta et al.
    Pages 169-180

  14. Murine Models for Lineage Tracing Cancer Initiating Cells

    • Kanchan Singh, Jennifer M. Bailey-Lundberg
    Pages 181-193

  15. Murine Model of Obesity-Induced Cancer

    • Niharika Badi, Zobeida Cruz-Monserrate
    Pages 195-201

  16. Lung Cancer Murine Models and Methodology for Immunopreventive Study

    • Marco Ramos-Castaneda, Seyed Javad Moghaddam
    Pages 203-214

  17. Mouse Models to Study Secondary Cancer Prevention

    • Le Li, Vidhi Chandra, Florencia McAllister, Yu Zhang
    Pages 215-223

  18. Back Matter

    Pages 225-227
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Keywords

About this book

This volume provides methods and techniques to further the study of cancer immunoprevention. Chapters describe tumor-associated antigens, cancer immune-preventive vaccines, generation of TILs, development of monoclonal antibodies, immunoprofiling technologies, tissue multispectral imaging techniques, mass cytometry on suspensions, mutiparametric flow cytometry, genomic expression analysis, and proteomic profiling of tumor microenvironment cell populations and metabolic assessment through novel imaging technologies.  Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Cancer Immunoprevention: Methods and Protocol aims to further understanding, development of interventional active strategies, and immune-interception of cancer.

Editors and Affiliations

  • The University of Texas MD Anderson Cancer Center, Houston, USA

    Florencia McAllister

Bibliographic Information

  • Book Title: Cancer Immunoprevention

  • Book Subtitle: Methods and Protocols

  • Editors: Florencia McAllister

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2014-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2022

  • Hardcover ISBN: 978-1-0716-2013-7Published: 07 January 2022

  • Softcover ISBN: 978-1-0716-2016-8Published: 08 January 2023

  • eBook ISBN: 978-1-0716-2014-4Published: 06 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 227

  • Number of Illustrations: 2 b/w illustrations, 35 illustrations in colour

  • Topics: Cancer Research, Immunology

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