Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2431)
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Table of contents (29 protocols)
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Front Matter
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Rodent Primary Neurons
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Front Matter
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Human-Derived Neurons
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Front Matter
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Keywords
About this book
This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron.
The chapters “ Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
Editors and Affiliations
Bibliographic Information
Book Title: Axonal Transport
Book Subtitle: Methods and Protocols
Editors: Alessio Vagnoni
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1990-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1989-6Published: 13 April 2022
Softcover ISBN: 978-1-0716-1992-6Published: 13 April 2022
eBook ISBN: 978-1-0716-1990-2Published: 12 April 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 573
Number of Illustrations: 19 b/w illustrations, 126 illustrations in colour
Topics: Neurobiology, Neurosciences