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Dengue Virus

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Ronaldo Mohana-Borges

    1. Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2409)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Virus and Virus Protein Production

    1. Front Matter

      Pages 1-1
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    2. Virus Propagation and Plaque Assay for Dengue Virus

      • Pedro Henrique Carneiro, Lucas Mendes-Monteiro, Ronaldo Mohana-Borges
      Pages 3-9

    3. Transmission Electron Microscopy Studies of Dengue Viruses

      • Gabriela Cardoso Caldas, Ortrud Monika Barth, Debora Ferreira Barreto-Vieira
      Pages 11-30

    4. Production of Properly Folded NS1 Protein in Bacterial Cells

      • Diego Allonso
      Pages 31-38

    5. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System

      • Diego R. Coelho, Pedro Henrique Carneiro
      Pages 39-46

    6. Purification of Dengue and Zika Virus Non-structural Protein 5 for Crystallization and Screening of Antivirals

      • Aicha Gharbi-Ayachi, Abbas El Sahili, Julien Lescar
      Pages 47-61

    7. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly

      • Szu-Chia Hsieh, Wen-Yang Tsai, Wei-Kung Wang
      Pages 63-76

    8. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons

      • Thu M. Cao, Richard J. Kuhn
      Pages 77-96

  3. Interaction Between Dengue Virus and Cellular Proteins

    1. Front Matter

      Pages 97-97
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    2. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis

      • Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, Laura C. Estrada
      Pages 99-117

    3. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions

      • Jonas Nascimento Conde
      Pages 119-132

    4. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry

      • Vivian Huerta, Yassel Ramos
      Pages 133-153

  4. Immunopathogensis and Diagnosis

    1. Front Matter

      Pages 155-155
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    2. Molecular Diagnosis of Dengue

      • Priscila C. G. Nunes, Monique R. Q. Lima, Flávia B. dos Santos
      Pages 157-171

    3. Serological Diagnosis of Dengue

      • Monique R. Q. Lima, Priscila C. G. Nunes, Flávia B. dos Santos
      Pages 173-196

    4. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies

      • Jonas Nascimento Conde, Megan Mladinich, William Schutt, Erich R. Mackow
      Pages 197-205

    5. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus

      • Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, Luciana Barros de Arruda
      Pages 207-222

    6. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells

      • Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, Guilherme Ferreira Silveira
      Pages 223-234

    7. In Vitro Generation of Human Antibody-Secreting Cells Through the Stimulation of PBMCs with Dengue Virus Particles

      • Vivian Bonezi, Allan Henrique Depieri Cataneo, Pryscilla Fanini Wowk, Eduardo L. V. Silveira
      Pages 235-244
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Keywords

About this book

This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.

Editors and Affiliations

  • Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Ronaldo Mohana-Borges

Bibliographic Information

  • Book Title: Dengue Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Ronaldo Mohana-Borges

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1879-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1878-3Published: 29 October 2021

  • Softcover ISBN: 978-1-0716-1881-3Published: 29 October 2022

  • eBook ISBN: 978-1-0716-1879-0Published: 28 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 295

  • Number of Illustrations: 9 b/w illustrations, 56 illustrations in colour

  • Topics: Virology, Immunology

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