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Enzyme Engineering

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Francesca Magnani

    1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Chiara Marabelli

    1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

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    You can also search for this editor in PubMed   Google Scholar

  3. Francesca Paradisi

    1. Department of Chemistry & Biochemistry, University of Bern, Bern, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2397)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Enzyme Libraries Preparation and Screening

    1. Front Matter

      Pages 1-1
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    2. Preparation of Soil Metagenome Libraries and Screening for Gene-Specific Amplicons

      • Luke J. Stevenson, David F. Ackerley, Jeremy G. Owen
      Pages 3-17

    3. Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics

      • Davide Agostino Cecchini, Mercedes Sánchez-Costa, Alejandro H. Orrego, Jesús Fernández-Lucas, Aurelio Hidalgo
      Pages 19-32

    4. Synthetic DNA Libraries for Protein Engineering Toward Process Improvement in Drug Synthesis

      • Michele Tavanti
      Pages 33-45

  3. Directed Evolution

    1. Front Matter

      Pages 47-47
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    2. In Silico Prediction Methods for Site-Saturation Mutagenesis

      • Ge Qu, Zhoutong Sun
      Pages 49-69

    3. Recombination of Compatible Substitutions by 2GenReP and InSiReP

      • Haiyang Cui, Mehdi D. Davari, Ulrich Schwaneberg
      Pages 71-81

  5. Rational Design

    1. Front Matter

      Pages 157-157
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    2. Rational-Design Engineering to Improve Enzyme Thermostability

      • Vinutsada Pongsupasa, Piyanuch Anuwan, Somchart Maenpuen, Thanyaporn Wongnate
      Pages 159-178

    3. Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents

      • Haiyang Cui, Markus Vedder, Ulrich Schwaneberg, Mehdi D. Davari
      Pages 179-202

    4. In Silico Engineering of Enzyme Access Tunnels

      • Alfonso Gautieri, Federica Rigoldi, Archimede Torretta, Alberto Redaelli, Emilio Parisini
      Pages 203-225

    5. Quantum-Mechanical/Molecular-Mechanical (QM/MM) Simulations for Understanding Enzyme Dynamics

      • Rimsha Mehmood, Heather J. Kulik
      Pages 227-248

    6. Computational Enzyme Design at Zymvol

      • Emanuele Monza, Victor Gil, Maria Fatima Lucas
      Pages 249-259Open Access
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Keywords

About this book

This book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and timely, Enzyme Engineering: Methods and Protocols is an ideal guide for both the novice and the veteran researcher interested in biocatalysis.

Chapter 13 is available open access under a CC BY 4.0 license.




Editors and Affiliations

  • Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Francesca Magnani, Chiara Marabelli

  • Department of Chemistry & Biochemistry, University of Bern, Bern, Switzerland

    Francesca Paradisi

Bibliographic Information

  • Book Title: Enzyme Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Francesca Magnani, Chiara Marabelli, Francesca Paradisi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1826-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1825-7Published: 24 November 2021

  • Softcover ISBN: 978-1-0716-1828-8Published: 25 November 2022

  • eBook ISBN: 978-1-0716-1826-4Published: 23 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 360

  • Number of Illustrations: 15 b/w illustrations, 69 illustrations in colour

  • Topics: Enzymology, Biotechnology, Chemistry/Food Science, general

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