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Mycobacterium ulcerans

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Gerd Pluschke

    1. Swiss Tropical and Public Health Institute, Basel, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  2. Katharina Röltgen

    1. Stanford School of Medicine Department of Pathology, Stanford University, Stanford, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2387)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Mycobacterium ulcerans Disease and Host Pathogen Interaction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Overview: Mycobacterium ulcerans Disease (Buruli Ulcer)

      • Katharina Röltgen, Gerd Pluschke
      Pages 3-6

    3. Immunohistochemistry: A Method to Analyze M. ulcerans Infected Skin Tissue

      • Marie-Thérèse Ruf
      Pages 7-15

    4. Primary Isolation of Mycobacterium ulcerans

      • Dorothy Yeboah-Manu, Adwoa Asante-Poku, Sammy Yaw Aboagye
      Pages 17-28

    5. Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance

      • Christopher J. Day, Jessica Poole, Gerd Pluschke, Michael P. Jennings
      Pages 29-40

    6. Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture

      • Mélanie Foulon, Estelle Marion, Laurent Marsollier
      Pages 41-51

    7. DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR

      • Caroline J. Lavender, Janet A. M. Fyfe
      Pages 53-62

    8. Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans

      • Janet A. M. Fyfe, Caroline J. Lavender
      Pages 63-69

    9. Detection of Mycobacterium ulcerans DNA Using Real-Time PCR

      • Janet A. M. Fyfe, Caroline J. Lavender
      Pages 71-80

    10. Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR

      • Marcus Beissner, Richard Odame Phillips, Gisela Bretzel
      Pages 81-86

    11. Methods and Approaches for Buruli Ulcer Surveillance in Africa: Lessons Learnt and Future Directions

      • Joseph W. S. Timothy, Rachel L. Pullan, Rie R. Yotsu
      Pages 87-102

  3. Purification and Quantification of Myolactone

    1. Front Matter

      Pages 103-103
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    2. Overview: Mycolactone , the Macrolide Toxin of Mycobacterium ulcerans

      • Katharina Röltgen, Gerd Pluschke
      Pages 105-108

    3. Conditions for Handling and Optimal Storage of Mycolactone

      • Jessica Z. Kubicek-Sutherland, Basil I. Swanson, Harshini Mukundan
      Pages 109-116

    4. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples

      • Aline Rifflet, Caroline Demangel, Laure Guenin-Macé
      Pages 117-130

    5. Detection of Mycolactone by Thin Layer Chromatography

      • Richard Kwamla Amewu, Thomas Spangenberg
      Pages 131-149

    6. Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone

      • Louisa Warryn
      Pages 151-161

    7. Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61

      • Sarah O’Keefe, Stephen High, Caroline Demangel
      Pages 163-181

  4. Drug Development Against Mycobacterium ulcerans

    1. Front Matter

      Pages 183-183
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Keywords

About this book

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

Editors and Affiliations

  • Swiss Tropical and Public Health Institute, Basel, Switzerland

    Gerd Pluschke

  • Stanford School of Medicine Department of Pathology, Stanford University, Stanford, USA

    Katharina Röltgen

Bibliographic Information

  • Book Title: Mycobacterium ulcerans

  • Book Subtitle: Methods and Protocols

  • Editors: Gerd Pluschke, Katharina Röltgen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1779-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1778-6Published: 14 October 2021

  • Softcover ISBN: 978-1-0716-1781-6Published: 14 October 2022

  • eBook ISBN: 978-1-0716-1779-3Published: 13 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 232

  • Number of Illustrations: 8 b/w illustrations, 25 illustrations in colour

  • Topics: Microbiology, Immunology, Pathology

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