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Long Non-Coding RNAs

Methods and Protocols

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Lin Zhang

    1. Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Xiaowen Hu

    1. Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible result
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2372)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-Associated Proteins

    • Zhen Xing, Sergey Egranov, Chunru Lin, Liuqing Yang
    Pages 1-10

  3. Crosslinking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions of Long Noncoding RNAs with Canonical and Non-canonical RNA-Binding Proteins

    • Jung-Hyun Cho, Ji Won Lee, Je-Hyun Yoon, Kyung-Won Min
    Pages 11-18

  4. Characterization of Long Non-coding RNA Associated Proteins by RNA-Immunoprecipitation

    • Junjie Jiang, Tianli Zhang, Yutian Pan, Zhongyi Hu, Jiao Yuan, Xiaowen Hu et al.
    Pages 19-26

  5. Isolation of Protein Complexes Associated with Long Non-coding RNAs

    • Min Li, Ying Xue, Ruiping He, Qihong Huang
    Pages 27-33

  6. Detection of Long Noncoding RNA Expression by Real-Time PCR

    • Rui Huang, Yihao Wang, Yaqi Deng, Jianfeng Shen
    Pages 35-42

  7. Profiling Long Non-coding RNA expression Using Custom-Designed Microarray

    • Xinna Zhang, George A. Calin
    Pages 43-51

  8. Long Non-coding RNA Expression Profiling Using Arraystar LncRNA Microarrays

    • Yanggu Shi, Jindong Shang
    Pages 53-74

  9. Nuclear RNA Isolation and Sequencing

    • Navroop K. Dhaliwal, Jennifer A. Mitchell
    Pages 75-83

  10. Capturing Endogenous Long Noncoding RNAs and Their Binding Proteins Using Chromatin Isolation by RNA Purification

    • Jongchan Kim, Li Ma
    Pages 85-92

  11. Purification and Structural Characterization of the Long Noncoding RNAs

    • Allison Yankey, Sean C. Clark, Michael C. Owens, Srinivas Somarowthu
    Pages 93-110

  12. Simultaneous RNA-DNA FISH

    • Lan-Tian Lai, Zhenyu Meng, Fangwei Shao, Li-Feng Zhang
    Pages 111-121

  13. Highly Resolved Detection of Long Non-coding RNAs In Situ

    • Megan Trotter, Clair Harris, Marissa Cloutier, Milan Samanta, Sundeep Kalantry
    Pages 123-144

  14. Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots

    • Yueying Wang, Mu Xu, Jiao Yuan, Zhongyi Hu, Youyou Zhang, Lin Zhang et al.
    Pages 145-156

  15. Assessment of In Vivo siRNA Delivery in Cancer Mouse Models

    • Lingegowda S. Mangala, Cristian Rodriguez-Aguayo, Emine Bayraktar, Nicholas B. Jennings, Gabriel Lopez-Berestein, Anil K. Sood
    Pages 157-168

  16. Genetic Editing of Long Noncoding RNA Using CRISPR/Cas9 Technology

    • Kristina Larter, Bin Yi, Yaguang Xi
    Pages 169-177

  17. Characterization of Circular RNAs

    • Yang Zhang, Li Yang, Ling-Ling Chen
    Pages 179-192

  18. Identification of circRNA-Interacting Proteins by Affinity Pulldown

    • Jen-Hao Yang, Poonam R. Pandey, Myriam Gorospe
    Pages 193-202

  19. Exploration of Circular RNA Interactomes by RNA Pull-Down Method

    • Mengshi Wu, Dan Peng, Xiaomin Zhong
    Pages 203-208

  20. Methods for Characterization of Alternative RNA Splicing

    • Samuel E. Harvey, Jingyi Lyu, Chonghui Cheng
    Pages 209-222
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Keywords

About this book

This second edition provides a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Long Non-Coding RNAs: Methods and Protocols,Second Edition aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, USA

    Lin Zhang, Xiaowen Hu

Bibliographic Information

  • Book Title: Long Non-Coding RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Lin Zhang, Xiaowen Hu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1697-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1696-3Published: 21 August 2021

  • Softcover ISBN: 978-1-0716-1699-4Published: 22 August 2022

  • eBook ISBN: 978-1-0716-1697-0Published: 20 August 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 308

  • Number of Illustrations: 7 b/w illustrations, 41 illustrations in colour

  • Topics: Gene Expression

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