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Protein Microarrays for Disease Analysis

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Rodrigo Barderas

    1. Functional Proteomics Unit, Instituto de Salud Carlos III, Madrid, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Joshua LaBaer

    1. Biodesign Institute, Arizona State University, Tempe, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Sanjeeva Srivastava

    1. Proteomics Laboratory, Indian Institute of Technology Bombay, Mumbai, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2344)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Protein Microarray-Based Proteomics for Disease Analysis

      • Rodrigo Barderas, Sanjeeva Srivastava, Joshua LaBaer
      Pages 3-6

  3. Design and Production of Protein Microarrays

    1. Front Matter

      Pages 7-7
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    2. Systematic and Rational Design of Protein Arrays in Noncontact Printers: Pipeline and Critical Aspects

      • Pablo Juanes-Velasco, Alicia Landeira-Viñuela, Angela-Patricia Hernandez, Manuel Fuentes
      Pages 9-29

    3. Phage Microarrays for Screening of Humoral Immune Responses

      • Ana Montero-Calle, Pablo San Segundo-Acosta, María Garranzo-Asensio, Guillermo Solís-Fernández, Maricruz Sanchez-Martinez, Rodrigo Barderas
      Pages 31-46

    4. Identification of Antibody Biomarker Using High-Density Nucleic Acid Programmable Protein Array

      • Lusheng Song, Peter Wiktor, Ji Qiu, Joshua LaBaer
      Pages 47-64

    5. Bead-Based Assays for Validating Proteomic Profiles in Body Fluids

      • Annika Bendes, Matilda Dale, Cecilia Mattsson, Tea Dodig-Crnković, Maria Jesus Iglesias, Jochen M. Schwenk et al.
      Pages 65-78

  4. Applications of Protein Microarrays

    1. Front Matter

      Pages 79-79
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    2. Analysis of Protein-Protein Interactions by Protein Microarrays

      • Ana Montero-Calle, Rodrigo Barderas
      Pages 81-97

    3. Detection of Posttranslational Modification Autoantibodies Using Peptide Microarray

      • Meng Li, Hongye Wang, Jiayu Dai, Meng Xu, Jianhua Liu, Jing Ren et al.
      Pages 99-106

    4. Epitope Mapping of Allergenic Lipid Transfer Proteins

      • Clara San Bartolomé, Carmen Oeo-Santos, Pablo San Segundo-Acosta, Rosa Muñoz-Cano, Javier Martínez-Botas, Joan Bartra et al.
      Pages 107-117

    5. Epitope Mapping of Food Allergens Using Noncontact Piezoelectric Microarray Printer

      • Javier Martínez-Botas, Carlos Fernández-Lozano, Alberto Rodríguez-Alonso, Laura Sánchez-Ruano, Belén de la Hoz
      Pages 119-135

  5. Deciphering Immune Responses in Diseases

    1. Front Matter

      Pages 137-137
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    2. Protein Arrays for the Identification of Seroreactive Protein Markers for Infectious Diseases

      • Apoorva Venkatesh, Aarti Jain, Huw Davies, Philip L. Felgner, Pradipsinh K. Rathod, Swati Patankar et al.
      Pages 139-150

    3. Glass Slide-Printed Protein Arrays as a Platform to Discover Serodiagnostic Antigens Against Bacterial Infections

      • Alfonso Olaya-Abril, Manuel J. Rodríguez-Ortega
      Pages 151-161

    4. Affinity Proteomics Assays for Cardiovascular and Atherosclerotic Disease Biomarkers

      • Maria Jesus Iglesias, Jochen M. Schwenk, Jacob Odeberg
      Pages 163-179

    5. Nucleic Acid Programmable Protein Arrays (NAPPA) for the Discovery of Autoantibodies in Osteoarthritis

      • Lucía Lourido, María Camacho-Encina, Francisco J. Blanco, Cristina Ruiz-Romero
      Pages 181-190

    6. Profiling Autoantibody Responses to Devise Novel Diagnostic and Prognostic Markers Using High-Density Protein Microarrays

      • Shabarni Gupta, Arghya Banerjee, Parvez Syed, Sanjeeva Srivastava
      Pages 191-208

  6. Antibody Microarrays for the Characterization of Disease-Specific Protein Profiles

    1. Front Matter

      Pages 209-209
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Keywords

About this book

This volume focuses on protein analysis, and covers a wide array of uses of protein microarray for disease analysis. The chapters in this book discuss different stages of protein microarrays from their construction to their use, including different types of protein microarrays such as recombinant proteins, antibody, phage, and NAPPA protein microarrays, in planar format or in solution via beads arrays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Protein Microarrays for Disease Analysis: Methods and Protocols is a valuable resource for graduate and post-doctoral fellows interested in protein microarrays, as well as senior researchers interested in gaining more insight into this developing field.


Editors and Affiliations

  • Functional Proteomics Unit, Instituto de Salud Carlos III, Madrid, Spain

    Rodrigo Barderas

  • Biodesign Institute, Arizona State University, Tempe, USA

    Joshua LaBaer

  • Proteomics Laboratory, Indian Institute of Technology Bombay, Mumbai, India

    Sanjeeva Srivastava

Bibliographic Information

  • Book Title: Protein Microarrays for Disease Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Rodrigo Barderas, Joshua LaBaer, Sanjeeva Srivastava

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1562-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1561-4Published: 12 June 2021

  • Softcover ISBN: 978-1-0716-1564-5Published: 11 June 2021

  • eBook ISBN: 978-1-0716-1562-1Published: 11 June 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 282

  • Number of Illustrations: 71 b/w illustrations

  • Topics: Protein Science

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