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Proteomic Profiling

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Anton Posch

    1. Bio-Rad Laboratories GmbH, Feldkirchen, Germany

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2261)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. How Modern Mass Spectrometry Can Solve Ancient Questions: A Multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi

    • Rudolf Grimm
    Pages 1-12

  3. Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis

    • Xinping Li, Thomas Franz, Ilian Atanassov, Thomas Colby
    Pages 13-23

  4. Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis

    • Yi-Han Lin, Rodrigo Vargas Eguez, Isha Vashee, Yanbao Yu
    Pages 25-34

  5. Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry

    • Ximo Zhang
    Pages 35-53

  6. Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation

    • Marcella Nunes Melo-Braga, María Ibáñez-Vea, Katarzyna Kulej, Martin R. Larsen
    Pages 55-72

  7. Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins

    • Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
    Pages 73-78

  8. Polyubiquitin Profile in Down Syndrome and Alzheimer’s Disease Brain

    • Antonella Tramutola, Marzia Perluigi
    Pages 79-91

  9. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing

    • Jiaqi Wu, Will McElroy, Charles Haitjema, Carsten Lück, Christopher Heger
    Pages 93-103

  10. A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes

    • Alin Rai, Haoyun Fang, Monique Fatmous, Bethany Claridge, Qi Hui Poh, Richard J. Simpson et al.
    Pages 105-149

  11. Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma

    • Antonia Reale, Tiffany Khong, Rong Xu, Maoshan Chen, Sridurga Mithraprabhu, Nicholas Bingham et al.
    Pages 151-191

  12. Isolation of Extracellular Vesicles for Proteomic Profiling

    • Dongsic Choi, Janusz Rak, Yong Song Gho
    Pages 193-206

  13. Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses

    • Prabal Subedi, Michael Schneider, Michael J. Atkinson, Soile Tapio
    Pages 207-212

  14. Flow Cytometry as an Important Tool in Proteomic Profiling

    • Michael P. Blundell, Sharon L. Sanderson, Tracey A. Long
    Pages 213-227

  15. Improved Immunoprecipitation to Mass Spectrometry Method for the Enrichment of Low-Abundant Protein Targets

    • Penny Jensen, Bhavin Patel, Suzanne Smith, Renuka Sabnis, Barbara Kaboord
    Pages 229-246

  16. Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors

    • Jonathan M. Preuss, Ute Burret, Sabine Vettorazzi
    Pages 247-262

  17. Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks

    • Candice Cox
    Pages 263-276

  18. Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma

    • Viktoria Anselm, Andreas Steinhilber, Cornelia Sommersdorf, Oliver Poetz
    Pages 277-289

  19. Recombinant Anti-idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development

    • Stefan Harth, Christian Frisch
    Pages 291-306

  20. cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection

    • Chathuni Jayathilake, Naoto Nemoto
    Pages 307-321
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Keywords

About this book

This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.

Editors and Affiliations

  • Bio-Rad Laboratories GmbH, Feldkirchen, Germany

    Anton Posch

Bibliographic Information

  • Book Title: Proteomic Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Anton Posch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1186-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1185-2Published: 10 January 2021

  • Softcover ISBN: 978-1-0716-1188-3Published: 10 January 2022

  • eBook ISBN: 978-1-0716-1186-9Published: 09 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 592

  • Number of Illustrations: 40 b/w illustrations, 91 illustrations in colour

  • Topics: Protein Science, Cell Biology

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