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Epigenetic Reprogramming During Mouse Embryogenesis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2214)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency

    • Katia Boutourlinsky, Nicolas Allègre, Claire Chazaud
    Pages 1-10
  3. Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos

    • Sandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, Michel Cohen-Tannoudji
    Pages 11-30
  4. Generation of Primordial Germ Cell-like Cells on Small and Large Scales

    • Wolfram H. Gruhn, Ufuk Günesdogan
    Pages 75-89
  5. Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions

    • Valentina Carlini, Kristjan H. Gretarsson, Jamie A. Hackett
    Pages 91-108
  6. Somatic Reprograming by Nuclear Transfer

    • Vincent Brochard, Nathalie Beaujean
    Pages 109-123
  7. Targeted Transgenic Mice Using CRISPR /Cas9 Technology

    • Fatima El Marjou, Colin Jouhanneau, Denis Krndija
    Pages 125-141
  8. Mapping of Chromosome Territories by 3D-Chromosome Painting During Early Mouse Development

    • Katia Ancelin, Yusuke Miyanari, Olivier Leroy, Maria-Elena Torres-Padilla, Edith Heard
    Pages 175-187
  9. Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-Seq

    • Raquel Pérez-Palacios, Patricia Fauque, Aurélie Teissandier, Déborah Bourc’his
    Pages 189-205
  10. Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals

    • Ambre Bender, Hala Al Adhami, Thomas Dahlet, Michael Weber
    Pages 207-220
  11. Profiling DNA Methylation Genome-Wide in Single Cells

    • António Galvão, Gavin Kelsey
    Pages 221-240
  12. Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN

    • Srinivasa Abishek Prakash, Joan Barau
    Pages 253-264
  13. DamID to Map Genome-Protein Interactions in Preimplantation Mouse Embryos

    • Mrinmoy Pal, Jop Kind, Maria-Elena Torres-Padilla
    Pages 265-282
  14. Understanding Chromosome Structure During Early Mouse Development by a Single-Cell Hi-C Analysis

    • Noémie Ranisavljevic, Maud Borensztein, Katia Ancelin
    Pages 283-293

About this book

This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming. 


Editors and Affiliations

  • Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Paris, France

    Katia Ancelin

  • Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Institut de Ge´ne´tique Mole´culaire de Montpellier, Univ Montpellier, CNRS Montpellier, France, Paris, France

    Maud Borensztein

Bibliographic Information

  • Book Title: Epigenetic Reprogramming During Mouse Embryogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Katia Ancelin, Maud Borensztein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0958-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0957-6Published: 19 September 2020

  • Softcover ISBN: 978-1-0716-0960-6Published: 18 September 2020

  • eBook ISBN: 978-1-0716-0958-3Published: 18 September 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 318

  • Number of Illustrations: 16 b/w illustrations, 58 illustrations in colour

  • Topics: Developmental Biology, Genetics and Genomics, Biological Techniques

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access