Vascular Morphogenesis

1. Front Matter

   Pages i-xi

   PDF

2. Historical Overview of In Vivo and In Vitro Angiogenesis Assays

   • Anca Maria Cimpean, Marius Raica

   Pages 1-13

3. The Fundamental Contribution of Judah Folkman in the Setting of Angiogenesis Assays

   • Domenico Ribatti

   Pages 15-25

4. Identification of Endothelial Cells and Their Progenitors

   • Ellen Go, Mervin C. Yoder

   Pages 27-37

5. In Vitro Coculture Assays of Angiogenesis

   • Haoche Wei, Ananthalakshmy Sundararaman, Sarah Line Bring Truelsen, David Gurevich, Jacob Thastrup, Harry Mellor

   Pages 39-46

6. Endothelial Cells: Co-culture Spheroids

   • Janos M. Kanczler, Julia A. Wells, Richard O. C. Oreffo

   Pages 47-56

7. Microfluidic Device Setting by Coculturing Endothelial Cells and Mesenchymal Stem Cells

   • Masafumi Watanabe, Ryo Sudo

   Pages 57-66

8. Spatial Statistics-Based Image Analysis Methods for the Study of Vascular Morphogenesis

   • Diego Guidolin, Cinzia Tortorella, Domenico Ribatti

   Pages 67-88

9. Studying Angiogenesis in the Rabbit Corneal Pocket Assay

   • Lucia Morbidelli, Valerio Ciccone, Marina Ziche

   Pages 89-101

10. Avians as a Model System of Vascular Development

    • Rieko Asai, Michael Bressan, Takashi Mikawa

    Pages 103-127

11. Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture

    • Andrew L. Lopez III, Irina V. Larina

    Pages 129-141

12. High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels

    • Mariella Errede, Francesco Girolamo, Daniela Virgintino

    Pages 143-150

13. Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels

    • Marco Righi, Marco Presta, Arianna Giacomini

    Pages 151-178

14. A Xenograft Model for Venous Malformation

    • Jillian Goines, Elisa Boscolo

    Pages 179-192

15. In Vivo Vascular Network Forming Assay

    • Hwan D. Kim, Ruei-Zeng Lin, Juan M. Melero-Martin

    Pages 193-203

16. Assessment of Vascular Patterning in the Zebrafish

    • Amber N. Stratman, Brant M. Weinstein

    Pages 205-222

17. By the Skin of Your Teeth: A Subcutaneous Mouse Model to Study Pulp Regeneration

    • Annelies Bronckaers, Petra Hilkens, Esther Wolfs, Ivo Lambrichts

    Pages 223-232

18. Back Matter

    Pages 233-234

    PDF

This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field. Of note, the book explores endothelial cell signaling is currently understood to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation, and postnatal tissue remodeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and timely, Vascular Morphogenesis: Methods and Protocols, Second Edition will serve as an ideal guide to this area of study for a broad audience amongst cell biologists, anatomists, pathologists, and physiologists.

• Endothelial cell signaling
• Blood vessel formation
• Vasculogenesis
• Vascular bed specificity
• Angiocrine factors

• Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, Bari, Italy

  Domenico Ribatti

Policies and ethics