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Wound Regeneration

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Hiranmoy Das

    1. Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center, Amarillo, USA

    View editor publications

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2193)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay

    • John L. Zemkewicz, Racheal G. Akwii, Constantinos M. Mikelis, Colleen L. Doçi
    Pages 1-11

  3. A Murine Incisional Fetal Wound-Healing Model to Study Scarless and Fibrotic Skin Repair

    • Traci A. Wilgus
    Pages 13-21

  4. Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp–Derived Stem Cell Product for Healing

    • Carl Greene, Hiranmoy Das
    Pages 23-30

  5. Musculoskeletal Tissue Engineering Using Fibrous Biomaterials

    • George Tan, Yingge Zhou, Dilshan Sooriyaarachchi
    Pages 31-40

  6. Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy

    • Sarah Anderson, Hiranmoy Das
    Pages 41-48

  7. Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact

    • Marie-Line Fournier, Tifenn Clément, Justine Aussudre, Nikolaus Plesnila, André Obenaus, Jérôme Badaut
    Pages 49-65

  8. Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models

    • Xi Li, Ning Xu Landén
    Pages 67-75

  9. Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism

    • A K M Nawshad Hossian, George Mattheolabakis
    Pages 77-83

  10. In Vivo Ear Sponge Lymphangiogenesis Assay

    • Racheal G. Akwii, Md S. Sajib, Fatema T. Zahra, Hanumantha R. Madala, Kalkunte S. Srivenugopal, Constantinos M. Mikelis
    Pages 85-96

  11. Identification of Rho GEF and RhoA Activation by Pull-Down Assays

    • Md S. Sajib, Fatema T. Zahra, Racheal G. Akwii, Constantinos M. Mikelis
    Pages 97-109

  12. Wound Matrix Stiffness Imposes on Macrophage Activation

    • Pu Duann, Pei-Hui Lin
    Pages 111-120

  13. Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ Hematopoietic Stem Cells

    • Derek Barthels, Hiranmoy Das
    Pages 121-128

  14. Electrospun Aligned Coaxial Nanofibrous Scaffold for Cardiac Repair

    • Divya Sridharan, Arunkumar Palaniappan, Britani N. Blackstone, Heather M. Powell, Mahmood Khan
    Pages 129-140

  15. Generation of Myocardial Ischemic Wounds and Healing with Stem Cells

    • Daniela Rolph, Hiranmoy Das
    Pages 141-147

  16. Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries

    • Peipei Pan, Matilda F. Chan
    Pages 149-158

  17. Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing

    • Arsia Jamali, Brendan M. Kenyon, Gustavo Ortiz, Betul N. Bayraktutar, Victor G. Sendra, Pedram Hamrah
    Pages 159-174

  18. Murine Corneal Epithelial Wound Modeling

    • Dhara Shah, Vinay Kumar Aakalu
    Pages 175-181

  19. Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors

    • Tejabhiram Yadavalli, Raghuram Koganti, Deepak Shukla
    Pages 183-196

  20. Back Matter

    Pages 197-207
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Keywords

About this book

This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields ofresearch.

Editors and Affiliations

  • Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center, Amarillo, USA

    Hiranmoy Das

Bibliographic Information

  • Book Title: Wound Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Hiranmoy Das

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0845-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0844-9Published: 19 August 2020

  • Softcover ISBN: 978-1-0716-0847-0Published: 19 August 2021

  • eBook ISBN: 978-1-0716-0845-6Published: 18 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 207

  • Number of Illustrations: 5 b/w illustrations, 47 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering, Biological Techniques, Stem Cells

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