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T-Cell Receptor Signaling

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2111)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. Exploration of T-Cell Diversity Using Mass Cytometry

    • Kaitlin C. O’Boyle, Takuya Ohtani, Sasikanth Manne, Bertram Bengsch, Sarah E. Henrickson, E. John Wherry et al.
    Pages 1-20
  3. A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells

    • Xian Jia, Xiaojuan Zhou, Haiping Zheng, Shan Jiang, Jiannan Weng, Lei Huang et al.
    Pages 21-33
  4. CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function

    • Wanjing Shang, Fei Wang, Qi Zhu, Liangyu Wang, Haopeng Wang
    Pages 59-70
  5. In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets

    • Jaclyn R. Espinosa, Joshua D. Wheaton, Maria Ciofani
    Pages 79-89
  6. CD4+ T-Cell Differentiation In Vitro

    • Wenyong Yang, Xueying Chen, Hongbo Hu
    Pages 91-99
  7. Primary T-Cell Transduction to Study Follicular Helper T-Cell Differentiation

    • Yang Zhang, Xuehui Long, Xiaoming Wang
    Pages 115-126
  8. In Vitro Generation of Stem Cell Memory-Like T Cells from Activated T Cells

    • Makoto Ando, Mari Ikeda, Akihiko Yoshimura, Taisuke Kondo
    Pages 127-139
  9. Artificial Antigen Presentosomes for T Cell Activation

    • Yi-Geng Pang, Chien-Chung Chang
    Pages 141-151
  10. Imaging Chimeric Antigen Receptor (CAR) Activation

    • Kendra A. Libby, Xiaolei Su
    Pages 153-160
  11. Testing the Efficiency and Kinetics of Negative Selection Using Thymic Slices

    • Tyng-An Zhou, Chia-Lin Hsu, Ivan Lilyanov Dzhagalov
    Pages 205-219
  12. An Integrated Strategy for Identifying Targets of Ubiquitin-Mediated Degradation in CD4+ T Cells

    • Natania S. Field, Claire E. O’Leary, Joseph M. Dybas, Hua Ding, Paula M. Oliver
    Pages 239-256

About this book

This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell’s, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.

Editors and Affiliations

  • Department of Pathogen Biology, Huazhong University of Science and Technology, Wuhan, China

    Chaohong Liu

Bibliographic Information

  • Book Title: T-Cell Receptor Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Chaohong Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0266-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0265-2Published: 14 January 2020

  • Softcover ISBN: 978-1-0716-0268-3Published: 14 January 2021

  • eBook ISBN: 978-1-0716-0266-9Published: 13 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 296

  • Number of Illustrations: 16 b/w illustrations, 48 illustrations in colour

  • Topics: Biochemistry, general, Immunology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access