Therapeutic Enzymes: Function and Clinical Implications

1. Front Matter

   Pages i-viii

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2. Production and Purification of Therapeutic Enzymes

   • M. Ângela Taipa, Pedro Fernandes, Carla C. C. R. de Carvalho

   Pages 1-24

3. Production of Therapeutic Enzymes by Lentivirus Transgenesis

   • María Celeste Rodríguez, Natalia Ceaglio, Sebastián Antuña, María Belén Tardivo, Marina Etcheverrigaray, Claudio Prieto

   Pages 25-54

4. Quality Control and Downstream Processing of Therapeutic Enzymes

   • David Gervais

   Pages 55-80

5. X-Ray Crystallography in Structure-Function Characterization of Therapeutic Enzymes

   • Anastassios C. Papageorgiou

   Pages 81-103

6. Application of Fluorescence in Studying Therapeutic Enzymes

   • Zhaoshuai Wang, Caihong Li, Yinan Wei

   Pages 105-114

7. Key Physicochemical Characteristics Influencing ADME Properties of Therapeutic Proteins

   • Xing Jing, Yan Hou, William Hallett, Chandrahas G. Sahajwalla, Ping Ji

   Pages 115-129

8. Stability of Therapeutic Enzymes: Challenges and Recent Advances

   • Shubhrima Ghosh, Shahenvaz Alam, Anurag S. Rathore, S. K. Khare

   Pages 131-150

9. Engineering Strategies for Oral Therapeutic Enzymes to Enhance Their Stability and Activity

   • Philipp Lapuhs, Gregor Fuhrmann

   Pages 151-172

10. Enzymes in Metabolic Anticancer Therapy

    • Maristella Maggi, Claudia Scotti

    Pages 173-199

11. Alkaline Phosphatase Replacement Therapy

    • Maria Luisa Bianchi, Silvia Vai

    Pages 201-232

12. Enzybiotics: Enzyme-Based Antibacterials as Therapeutics

    • Dorien Dams, Yves Briers

    Pages 233-253

13. Clinical Applications of Hyaluronidase

    • Gregor Cornelius Weber, Bettina Alexandra Buhren, Holger Schrumpf, Johannes Wohlrab, Peter Arne Gerber

    Pages 255-277

14. Alkaline Phosphatase Replacement Therapy for Hypophosphatasia in Development and Practice

    • S. A. Bowden, B. L. Foster

    Pages 279-322

15. Rational Use of Pancreatic Enzymes for Pancreatic Insufficiency and Pancreatic Pain

    • Gyanprakash A. Ketwaroo, David Y. Graham

    Pages 323-343

16. Fibrinolytic Enzymes for Thrombolytic Therapy

    • Swaroop S. Kumar, Abdulhameed Sabu

    Pages 345-381

17. Back Matter

    Pages 383-386

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Therapeutic enzymes exhibit fascinating features and opportunities, and represent a significant and promising subcategory of modern biopharmaceuticals for the treatment of several severe diseases. Research and drug developments efforts and the advancements in biotechnology over the past twenty years have greatly assisted the introduction of efficient and safe enzyme-based therapies for a range of both rare and common disorders. The introduction and regulatory approval of twenty different recombinant enzymes has enabled effective enzyme-replacement therapy. This volume aims to overview these therapeutic enzymes, focusing in particular on more recently approved enzymes produced by recombinant DNA technology. This volume is composed of four sections. Section 1 provides an overview of the production process and biochemical characterization of therapeutic enzymes, while Section 2 focuses upon the engineering strategies and delivery methods of therapeutic enzymes. Section 3 highlights the clinical applications of approved therapeutic enzymes, including aspects on their structure, indications and mechanisms of action. Together with information on these mechanisms, safety and immunogenicity issues and various adverse events of the recombinant enzymes used for therapy are discussed. Section 4, provides discussion on the prospective and future developments of new therapeutic enzymes. This book is aimed at academics, researchers and students undertaking advanced undergraduate/postgraduate programs in the biopharmaceutical/biotechnology area who wish to gain a comprehensive understanding of enzyme-based therapeutic molecules.

• Therapeutic Enzymes
• Recombinant Enzymes
• Protein Engineering
• Enzyme Therapy
• Enzyme Manufacturing

• Department of Biotechnology, Agricultural University of Athens, Athens, Greece

  Nikolaos Labrou

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