Editors:
- Offers a comprehensive view on directed evolution in the biotechnological ground
- Presents a state of the art summary of the methodical advances in this thrilling research field
- Highlights the most significant applications of engineered enzymes in industry
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Table of contents (10 chapters)
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Front Matter
About this book
This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art.
Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature.
The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the book summarizes a variety of the most applicable methods for library creation, together with the future trends aimed at bringing together directed evolution and in silico/computational enzyme design and ancestral resurrection.
Editors and Affiliations
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Department of Biocatalysis, Institute of Catalysis and Petrochemistry, CSIC, Madrid, Spain
Miguel Alcalde
About the editor
Bibliographic Information
Book Title: Directed Enzyme Evolution: Advances and Applications
Editors: Miguel Alcalde
DOI: https://doi.org/10.1007/978-3-319-50413-1
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing AG 2017
Hardcover ISBN: 978-3-319-50411-7Published: 23 February 2017
Softcover ISBN: 978-3-319-84395-7Published: 15 July 2018
eBook ISBN: 978-3-319-50413-1Published: 14 February 2017
Edition Number: 1
Number of Pages: VII, 284
Number of Illustrations: 36 b/w illustrations, 26 illustrations in colour
Topics: Protein Science, Biochemical Engineering, Applied Microbiology, Bioinformatics