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Single Cell Metabolism

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Bindesh Shrestha

    1. Waters Corporation, Milford, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2064)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Single-Cell Metabolomics by Mass Spectrometry

    • Bindesh Shrestha
    Pages 1-8

  3. Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry

    • Linwen Zhang, Linda L. Allworth, Akos Vertes
    Pages 9-18

  4. Analysis of Lipids in Single Cells and Organelles Using Nanomanipulation-Coupled Mass Spectrometry

    • Mandy S. Phelps, Guido F. Verbeck
    Pages 19-30

  5. Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis

    • Zhenwei Wei, Xiaochao Zhang, Xingyu Si, Xiaoyun Gong, Sichun Zhang, Xinrong Zhang
    Pages 31-59

  6. Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells

    • Ning Pan, Wei Rao, Zhibo Yang
    Pages 61-71

  7. Applications of MicroArrays for Mass Spectrometry (MAMS) in Single-Cell Metabolomics

    • Alfredo J. Ibáñez, Ales Svatos
    Pages 73-88

  8. Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells

    • John F. Cahill, Vilmos Kertesz
    Pages 89-101

  9. Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

    • Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, Bernhard Spengler
    Pages 103-111

  10. A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population

    • Jasmin Krismer, Jens Sobek, Robert F. Steinhoff, Rolf Brönnimann, Martin Pabst, Renato Zenobi
    Pages 113-124

  11. Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometry

    • Bo Yang, Tina Tsui, Richard M. Caprioli, Jeremy L. Norris
    Pages 125-134

  12. Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry

    • Sylwia A. Stopka, Akos Vertes
    Pages 135-146

  13. Compact Quantum Dots for Quantitative Cytology

    • Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, Andrew M. Smith
    Pages 147-158

  14. Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos Using Desorption Electrospray Ionization (DESI) Mass Spectrometry

    • Christina R. Ferreira, Valentina Pirro, Alan K. Jarmusch, Clint M. Alfaro, R. Graham Cooks
    Pages 159-179

  15. Spatial Mapping of Cellular Metabolites Using DESI Ion Mobility Mass Spectrometry

    • Anthony Midey, Hernando Olivos, Bindesh Shrestha
    Pages 181-190

  16. Open-Source Software Tools, Databases, and Resources for Single-Cell and Single-Cell-Type Metabolomics

    • Biswapriya B. Misra
    Pages 191-217

  17. Ten Major Future Challenges in Single-Cell Metabolomics

    • Bindesh Shrestha
    Pages 219-223

  18. Back Matter

    Pages 225-226
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Keywords

About this book

This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of  introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field. 


Editors and Affiliations

  • Waters Corporation, Milford, USA

    Bindesh Shrestha

Bibliographic Information

  • Book Title: Single Cell Metabolism

  • Book Subtitle: Methods and Protocols

  • Editors: Bindesh Shrestha

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9831-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9829-6Published: 30 September 2019

  • eBook ISBN: 978-1-4939-9831-9Published: 29 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 226

  • Number of Illustrations: 10 b/w illustrations, 64 illustrations in colour

  • Topics: Cell Biology, Metabolomics

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