Lipid-Protein Interactions

1. Front Matter

   Pages i-xiv

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2. Multiscale Modeling and Simulation Approaches to Lipid–Protein Interactions

   • Roland G. Huber, Timothy S. Carpenter, Namita Dube, Daniel A. Holdbrook, Helgi I. Ingólfsson, William A. Irvine et al.

   Pages 1-30

3. Quartz Crystal Microbalances as Tools for Probing Protein–Membrane Interactions

   • Søren B. Nielsen, Daniel E. Otzen

   Pages 31-52

4. Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes

   • Aleksandra Šakanovič, Vesna Hodnik, Gregor Anderluh

   Pages 53-70

5. Thermodynamic Analysis of Protein–Lipid Interactions by Isothermal Titration Calorimetry

   • Musti J. Swamy, Rajeshwer S. Sankhala, Bhanu Pratap Singh

   Pages 71-89

6. Differential Scanning Calorimetry of Protein–Lipid Interactions

   • Olga Cañadas, Cristina Casals

   Pages 91-106

7. Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope

   • K. Tanuj Sapra

   Pages 107-144

8. Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel Electrophoresis

   • Andre Schüßler, Sascha Herwig, Jörg H. Kleinschmidt

   Pages 145-162

9. Optimized Negative-Staining Protocol for Lipid–Protein Interactions Investigated by Electron Microscopy

   • Jianfang Liu, Hao Wu, Changyu Huang, Dongsheng Lei, Meng Zhang, Wei Xie et al.

   Pages 163-173

10. Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry

    • John W. Patrick, Arthur Laganowsky

    Pages 175-190

11. Protein Microarrays and Liposome: A Method for Studying Lipid–Protein Interactions

    • Samuel Herianto, Chien-Sheng Chen, Heng Zhu

    Pages 191-199

12. Structural Investigations of Protein–Lipid Complexes Using Neutron Scattering

    • Luke A. Clifton, Stephen C. L. Hall, Najet Mahmoudi, Timothy J. Knowles, Frank Heinrich, Jeremy H. Lakey

    Pages 201-251

13. Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment

    • Koichi Matsuo, Kunihiko Gekko

    Pages 253-279

14. FTIR Analysis of Proteins and Protein–Membrane Interactions

    • Suren A. Tatulian

    Pages 281-325

15. UV Resonance Raman Spectroscopy as a Tool to Probe Membrane Protein Structure and Dynamics

    • DeeAnn K. Asamoto, Judy E. Kim

    Pages 327-349

16. Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching

    • Gregory A. Caputo, Erwin London

    Pages 351-368

17. Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity

    • Luís M. S. Loura, Manuel Prieto, Fábio Fernandes

    Pages 369-382

18. A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers

    • Evan L. Taylor, Kumud Raj Poudel, James A. Brozik

    Pages 383-414

19. Fluorescence Correlation Spectroscopy to Examine Protein–Lipid Interactions in Membranes

    • Viktoria Betaneli, Jonas Mücksch, Petra Schwille

    Pages 415-447

20. Membrane Pore Formation by Peptides Studied by Fluorescence Techniques

    • Suren A. Tatulian, Nabin Kandel

    Pages 449-464

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications. Additional protocols were added to examine lipid-protein interactions by mass spectrometry, to use protein microarrays to investigate large sets of various proteins, to study membrane protein dynamics by UV resonance Raman spectroscopy, to analyze peptide-induced pore formation in membranes, and to investigate folding and insertion of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Second Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.

• lipidome scales
• fluorescence microscopy
• X-ray crystallography
• Protein–Membrane Interactions
• NMR Spectroscopy

• Department of Biophysics, Institute of Biology, FB10 and Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Kassel, Germany

  Jörg H. Kleinschmidt

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