- Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
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- About this book
-
This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.
- Table of contents (17 chapters)
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Detection of Cholera Toxin by an Immunochromatographic Test Strip
Pages 1-7
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Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins
Pages 9-23
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A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis
Pages 25-36
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Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin
Pages 37-47
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Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food
Pages 49-59
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Table of contents (17 chapters)
- Download Preface 1 PDF (29.9 KB)
- Download Sample pages 1 PDF (2 MB)
- Download Table of contents PDF (36.3 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Microbial Toxins
- Book Subtitle
- Methods and Protocols
- Editors
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- Otto Holst
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1600
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-6958-6
- DOI
- 10.1007/978-1-4939-6958-6
- Hardcover ISBN
- 978-1-4939-6956-2
- Softcover ISBN
- 978-1-4939-8351-3
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XI, 201
- Number of Illustrations
- 43 b/w illustrations, 28 illustrations in colour
- Topics
