Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1250)
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Table of contents (30 protocols)
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Front Matter
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In Vitro Models of the Hepatocyte Life Cycle
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Front Matter
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In Vitro Methods to Probe Hepatocyte Functionality and Toxicity
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Front Matter
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About this book
This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings. Â
Editors and Affiliations
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Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium
Mathieu Vinken, Vera Rogiers
Bibliographic Information
Book Title: Protocols in In Vitro Hepatocyte Research
Editors: Mathieu Vinken, Vera Rogiers
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2074-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2073-0Published: 19 November 2014
Softcover ISBN: 978-1-4939-4932-8Published: 22 September 2016
eBook ISBN: 978-1-4939-2074-7Published: 23 January 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 406
Number of Illustrations: 46 b/w illustrations, 26 illustrations in colour
Topics: Pharmacology/Toxicology