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Protocols in In Vitro Hepatocyte Research

  • Book
  • © 2015

Overview

Editors:
  1. Mathieu Vinken

    1. Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Vera Rogiers

    1. Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1250)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
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  2. In Vitro Models of the Hepatocyte Life Cycle

    1. Front Matter

      Pages 1-1
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    2. Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments

      • Lyndsey Houseman, Mina Edwards, Ian R. Phillips, Elizabeth A. Shephard
      Pages 3-12

    3. Cryopreservation of Hepatocytes

      • Nicola J. Hewitt, Albert P. Li
      Pages 13-26

    4. Culture Conditions Promoting Hepatocyte Proliferation and Cell Cycle Synchronization

      • Anne Corlu, Pascal Loyer
      Pages 27-51

    5. Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes

      • Eva Ramboer, Tamara Vanhaecke, Vera Rogiers, Mathieu Vinken
      Pages 53-76

    6. Culture and Functional Characterization of Human Hepatoma HepG2 Cells

      • María Teresa Donato, Laia Tolosa, María José Gómez-Lechón
      Pages 77-93

    7. Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death

      • Mathieu Vinken, Michaël Maes, Sara Crespo Yanguas, Joost Willebrords, Tamara Vanhaecke, Vera Rogiers
      Pages 95-103

    8. Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes

      • Kate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, David C. Hay
      Pages 105-111

    9. Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation

      • Joery De Kock, Robim M. Rodrigues, Karolien Buyl, Tamara Vanhaecke, Vera Rogiers
      Pages 113-122

    10. Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling

      • Richard L. Gieseck III, Ludovic Vallier, Nicholas R. F. Hannan
      Pages 123-142

    11. Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation

      • Varvara Gouliarmou, Olavi Pelkonen, Sandra Coecke
      Pages 143-159

    12. Coculture and Long-Term Maintenance of Hepatocytes

      • Merav Cohen, Gahl Levy, Yaakov Nahmias
      Pages 161-173

    13. Primary Hepatocytes in Sandwich Culture

      • Janneke Keemink, Marlies Oorts, Pieter Annaert
      Pages 175-188

    14. Establishing Liver Bioreactors for In Vitro Research

      • Sofia P. Rebelo, Rita Costa, Marcos F. Q. Sousa, Catarina Brito, Paula M. Alves
      Pages 189-202

    15. Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture

      • Jennifer Bolleyn, Joanna Fraczek, Vera Rogiers, Tamara Vanhaecke
      Pages 203-211

    16. Transfection of Primary Hepatocytes with Liver-Enriched Transcription Factors Using Adenoviral Vectors

      • Marta Benet, Ramiro Jover, Roque Bort
      Pages 213-221

  3. In Vitro Methods to Probe Hepatocyte Functionality and Toxicity

    1. Front Matter

      Pages 223-223
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    2. Transcriptomics of Hepatocytes Treated with Toxicants for Investigating Molecular Mechanisms Underlying Hepatotoxicity

      • Vaibhav Shinde, Regina Stöber, Harshal Nemade, Isaia Sotiriadou, Jürgen Hescheler, Jan Hengstler et al.
      Pages 225-240

    3. Global MicroRNA Analysis in Primary Hepatocyte Cultures

      • Julian Krauskopf, Almudena Espín-Pérez, Jos C. Kleinjans, Theo M. de Kok
      Pages 241-250
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Keywords

About this book

This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.  

Editors and Affiliations

  • Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium

    Mathieu Vinken, Vera Rogiers

Bibliographic Information

  • Book Title: Protocols in In Vitro Hepatocyte Research

  • Editors: Mathieu Vinken, Vera Rogiers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2074-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2073-0Published: 19 November 2014

  • Softcover ISBN: 978-1-4939-4932-8Published: 22 September 2016

  • eBook ISBN: 978-1-4939-2074-7Published: 23 January 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 406

  • Number of Illustrations: 46 b/w illustrations, 26 illustrations in colour

  • Topics: Pharmacology/Toxicology

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