Protocols in Advanced Genomics and Allied Techniques

1. Front Matter

   Pages i-xxix

   PDF

2. Conventional and Basic Genomic Techniques

   • Aruna Pal

   Pages 1-29

3. Recombinant DNA Technology

   • Aruna Pal

   Pages 31-47

4. Mitochondrial Genetics

   • Aruna Pal

   Pages 49-80

5. Cloning

   • Aruna Pal

   Pages 81-93

6. Immunohistochemistry

   • Aruna Pal

   Pages 95-117

7. Western Blotting

   • Aruna Pal

   Pages 119-127

8. Introduction to Bioinformatics

   • Aruna Pal

   Pages 129-158

9. Molecular Evolutionary Study: Phylogenetic Tree

   • Aruna Pal

   Pages 159-180

10. Real-Time or Quantitative PCR

    • Aruna Pal

    Pages 181-209

11. Tissue or Cell Cultures

    • Aruna Pal

    Pages 211-220

12. DNA Microarray

    • Aruna Pal

    Pages 221-243

13. Epigenetics and DNA Methylation

    • Aruna Pal

    Pages 245-278

14. RNA Sequencing (RNA-seq)

    • Aruna Pal

    Pages 279-322

15. Next-Generation Sequencing

    • Aruna Pal

    Pages 323-333

16. Metagenomics

    • Aruna Pal

    Pages 335-363

17. Genome-Wide Association Studies/SNP Chips

    • Aruna Pal

    Pages 365-392

18. Exome Sequencing

    • Aruna Pal

    Pages 393-419

19. RNA Interference

    • Aruna Pal

    Pages 421-448

20. Expressing Cloned Genes for Protein Production, Purification, and Analysis-Biopharming

    • Aruna Pal

    Pages 449-482

This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.

• genomics
• next generation sequencing
• gene therapy
• biological tools
• nutrigenomics
• bioinformatics
• DNA

• Animal Genetics and Breeding, West Bengal University of Animal and Fishery Sciences, Kolkata, India

  Aruna Pal

Dr Aruna Pal is an Assistant Professor at West Bengal University of Animal Fishery Sciences, India. She has served as a Scientist & Convener, Human Genetics Task Force at the Department of Biotechnology, Ministry of Science & Technology, Govt. of India. She has also worked as a Veterinary Officer at the Govt. of West Bengal and headed the Quality Control Laboratory, Frozen Semen Bull Station, PBGSBS (under the Central Project for Cattle Buffalo Breeding Programme, Ministry of Agriculture, Govt. of India).  She was awarded a Junior Research Fellowship (Indian Council of Agricultural Research) for pursuing her M.V.Sc, and a Senior Research Fellowship (Indian Veterinary Research Institute) for pursuing her Ph.D.  She has published 21 research papers in highly reputed journals, 2 books, 6 book chapters, 66 gene bank accession numbers (NCBI) and 18 research reviews. A pioneer in the documentation of new breeds of sheep, buffalo and duck from the State of West Bengal, India, she isalso an active member of nine scientific societies. She has been the Principal Investigator and co-Principal Investigator for three and six research projects, respectively.

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