Skip to main content
SpringerLink
Log in

Vascular Tissue Engineering

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Feng Zhao

    1. Department of Biomedical Engineering, Texas A&M University, College Station, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Kam W. Leong

    1. Department of Biomedical Engineering, Columbia University, New York, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2375)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling

    • Taylor Bertucci, Shravani Kakarla, Diana Kim, Guohao Dai
    Pages 1-12

  3. Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration

    • Randall J. Smith Jr, Stelios T. Andreadis
    Pages 13-19

  4. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells

    • Mei-Lan Li, Jiesi Luo, Matthew W. Ellis, Muhammad Riaz, Yasmeen Ajaj, Yibing Qyang
    Pages 21-34

  5. On-Site Differentiation of Human Mesenchymal Stem Cells into Vascular Cells on Extracellular Matrix Scaffold Under Mechanical Stimulations for Vascular Tissue Engineering

    • Na Li, Alex P. Rickel, Zhongkui Hong
    Pages 35-46

  6. End-Point Immobilization of Heparin on Electrospun Polycarbonate-Urethane Vascular Graft

    • Jun Fang, Song Li
    Pages 47-59

  7. Microfluidic Coaxial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits

    • Sushila Maharjan, Jacqueline Jialu He, Li Lv, Di Wang, Yu Shrike Zhang
    Pages 61-75

  8. In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System

    • Xu Zhang, Nadia O. Abutaleb, Ellen Salmon, George A. Truskey
    Pages 77-90

  9. Peritoneal Pre-conditioning Method for In Vivo Vascular Graft Maturation Utilizing a Porous Pouch

    • Mahyar Sameti, Chris A. Bashur
    Pages 91-99

  10. Fabrication of a Completely Biological and Anisotropic Human Mesenchymal Stem Cell-Based Vascular Graft

    • Weilue He, Dhavan Sharma, Wenkai Jia, Feng Zhao
    Pages 101-114

  11. Engineering Vascular Grafts with Multiphase Structures

    • Bryan D. James, Josephine B. Allen
    Pages 115-124

  12. Fabrication of Small-Diameter Tubular Grafts for Vascular Tissue Engineering Applications Using Mulberry and Non-mulberry Silk Proteins

    • Prerak Gupta, Biman B. Mandal
    Pages 125-139

  13. Fabrication and Evaluation of Tissue-Engineered Vascular Grafts with Hybrid Fibrous Structure

    • He Wang, Qiang Zhao
    Pages 141-152

  14. Controlling Pore Size of Electrospun Vascular Grafts by Electrospraying of Poly(Ethylene Oxide) Microparticles

    • Muhammad Rafique, Adam C. Midgley, Tingting Wei, Lianyong Wang, Deling Kong, Kai Wang
    Pages 153-164

  15. Injectable Hydrogels for Vascular Tissue Engineering

    • Fengqiao Li, William Ho, Xiaoyang Xu
    Pages 165-176

  16. Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis

    • Yuan Yao, YeJin Jeong, Aung Moe Zaw, Marek Kukumberg, Evelyn K. F. Yim
    Pages 177-189

  17. Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners

    • Aung Moe Zaw, Richard Shangguan, Yuan Yao, YeJin Jeong, Billy Y. S. Yiu, Adrian J. Y. Chee et al.
    Pages 191-201

  18. Vascular Graft Implantation Using a Bilateral End-to-Side Aortoiliac Preclinical Model

    • Khanh P. Nguyen, Monica T. Hinds, Deirdre E. J. Anderson
    Pages 203-215

  19. Quantification of In Vitro Blood-Brain Barrier Permeability

    • Bingmei M. Fu
    Pages 217-228

  20. Subcellular Force Quantification of Endothelial Cells Using Silicone Pillar Arrays

    • Mohanish K. Chandurkar, Sangyoon J. Han
    Pages 229-245
Back to top

Keywords

About this book

This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses. 


Editors and Affiliations

  • Department of Biomedical Engineering, Texas A&M University, College Station, USA

    Feng Zhao

  • Department of Biomedical Engineering, Columbia University, New York, USA

    Kam W. Leong

Bibliographic Information

  • Book Title: Vascular Tissue Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Feng Zhao, Kam W. Leong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1708-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1707-6Published: 01 October 2021

  • Softcover ISBN: 978-1-0716-1710-6Published: 02 October 2022

  • eBook ISBN: 978-1-0716-1708-3Published: 30 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 262

  • Number of Illustrations: 8 b/w illustrations, 71 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering, Physiology, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation