Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2353)
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Table of contents (17 protocols)
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Front Matter
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Methods for Genetic Manipulation and Fe-S Protein Expression
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Front Matter
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Biochemical Methods to Probe Steps Involved in the Synthesis of Fe-S Clusters
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Front Matter
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Biophysical Methods to Study Fe-S Clusters and Reactions Promoted by Metalloenzymes
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Front Matter
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About this book
This volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Fe-S Proteins: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
Editors and Affiliations
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Department of Chemistry, Wake Forest University, Winston Salem, USA
Patricia C. Dos Santos
Bibliographic Information
Book Title: Fe-S Proteins
Book Subtitle: Methods and Protocols
Editors: Patricia C. Dos Santos
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1605-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1604-8Published: 23 July 2021
Softcover ISBN: 978-1-0716-1607-9Published: 24 July 2022
eBook ISBN: 978-1-0716-1605-5Published: 22 July 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 351
Number of Illustrations: 23 b/w illustrations, 64 illustrations in colour
Topics: Protein Science