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Yarrowia lipolytica

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ian Wheeldon

    1. Chemical and Environmental Engineering, University of California Riverside, Riverside, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Mark Blenner

    1. Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2307)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Genome Engineering of Yarrowia lipolytica with the PiggyBac Transposon System

    • James M. Wagner, Claire M. Palmer, Maya V. Venkataraman, Lars H. Lauffer, Joshua M. Wiggers, Eden V. Williams et al.
    Pages 1-24

  3. Genome Editing in Y. lipolytica Using TALENs

    • Coraline Rigouin, Christian Croux, Gwendoline Dubois, Fayza Daboussi, Florence Bordes
    Pages 25-39

  4. EasyCloneYALI: Toolbox for CRISPR-Mediated Integrations and Deletions in Yarrowia lipolytica

    • Jonathan Dahlin, Carina Holkenbrink, Irina Borodina
    Pages 41-68

  5. Simultaneous Gene Excision and Integration by Dual-Guide CRISPR-Cas9

    • Michael Spagnuolo, Mark Blenner
    Pages 69-83

  6. Gene Excision by Dual-Guide CRISPR-Cas9

    • Michael Spagnuolo, Mark Blenner
    Pages 85-94

  7. CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica

    • Joshua Misa, Cory Schwartz
    Pages 95-109

  8. Implementing CRISPR-Cas12a for Efficient Genome Editing in Yarrowia lipolytica

    • Zhiliang Yang, Peng Xu
    Pages 111-121

  9. Guide RNA Design for Genome-Wide CRISPR Screens in Yarrowia lipolytica

    • Adithya Ramesh, Ian Wheeldon
    Pages 123-137

  10. Targeted Integration Through Transformation of Hydroxyurea-Arrested Cells

    • Vasiliki Tsakraklides
    Pages 139-145

  11. Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica

    • Erin L. Bredeweg, Scott E. Baker
    Pages 147-157

  12. Engineering Yarrowia lipolytica for Production of Fatty Alcohols with YaliBrick Vectors

    • Wanqi Sun, Zhiliang Yang, Peng Xu
    Pages 159-173

  13. Methods to Activate and Elucidate Complex Endogenous Sugar Metabolism in Yarrowia lipolytica

    • Seunghyun Ryu, Cong T. Trinh
    Pages 175-189

  14. Pathway Engineering for Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica

    • Mauricio Pesantes-Munoz, Rodrigo Ledesma-Amaro
    Pages 191-204

  15. Droplet-Based Microfluidic High-Throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica

    • Thomas Beneyton, Tristan Rossignol
    Pages 205-219

  16. Yarrowia lipolytica Bioprocess Development: From Flask to Bioreactor

    • Farshad Darvishi, Marzieh Moradi
    Pages 221-232

  17. Western Blotting of Membrane-Bound Proteins in Yarrowia lipolytica

    • Adithya Ramesh, Ian Wheeldon
    Pages 233-247

  18. Back Matter

    Pages 249-250
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Keywords

About this book

This volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica. Chapters detail the basic theories underlying the methods described in each chapter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Yarrowia lipolytica: Methods and Protocols aims to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully.


Editors and Affiliations

  • Chemical and Environmental Engineering, University of California Riverside, Riverside, USA

    Ian Wheeldon

  • Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, USA

    Mark Blenner

Bibliographic Information

  • Book Title: Yarrowia lipolytica

  • Book Subtitle: Methods and Protocols

  • Editors: Ian Wheeldon, Mark Blenner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1414-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1413-6Published: 14 April 2021

  • Softcover ISBN: 978-1-0716-1416-7Published: 15 April 2022

  • eBook ISBN: 978-1-0716-1414-3Published: 13 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 250

  • Number of Illustrations: 21 b/w illustrations, 44 illustrations in colour

  • Topics: Human Genetics

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