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Structure and Function of Membrane Proteins

  • Book
  • © 2021

Overview

Editors:
  1. Ingeborg Schmidt-Krey

    1. School of Biological Sciences and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. James C. Gumbart

    1. School of Physics, Georgia Institute of Technology, Atlanta, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2302)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL

    • Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, M. Joanne Lemieux
    Pages 1-20

  3. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies

    • Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, Ingeborg Schmidt-Krey
    Pages 21-35

  4. Biochemical Characterization of GPCR–G Protein Complex Formation

    • Filip Pamula, Ching-Ju Tsai
    Pages 37-48

  5. Electrophysiological Approaches for the Study of Ion Channel Function

    • Guiying Cui, Kirsten A. Cottrill, Nael A. McCarty
    Pages 49-67

  6. Isothermal Titration Calorimetry of Membrane Proteins

    • Han N. Vu, Alan J. Situ, Tobias S. Ulmer
    Pages 69-79

  7. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level

    • Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S. Chandler, Brendan P. Marsh, Gavin M. King
    Pages 81-99

  8. Structure Determination of Membrane Proteins Using X-Ray Crystallography

    • Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, Nicholas Noinaj
    Pages 101-136

  9. Studying Membrane Protein Structures by MicroED

    • Michael W. Martynowycz, Tamir Gonen
    Pages 137-151

  10. Single-Particle Cryo-EM of Membrane Proteins

    • Dovile Januliene, Arne Moeller
    Pages 153-178

  11. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy

    • Mary D. Hernando, Joseph O. Primeau, Howard S. Young
    Pages 179-199

  12. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel

    • Robert Silvers, Matthew T. Eddy
    Pages 201-217

  13. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering

    • Yuqi Wu, Kevin L. Weiss, Raquel L. Lieberman
    Pages 219-235

  14. Preparing Membrane Proteins for Simulation Using CHARMM-GUI

    • Yupeng Li, Jinchan Liu, James C. Gumbart
    Pages 237-251

  15. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide

    • William G. Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways et al.
    Pages 253-273

  16. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins

    • Yandong Huang, Jack A. Henderson, Jana Shen
    Pages 275-287

  17. Molecular Dynamics–Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions

    • Dylan Ogden, Mahmoud Moradi
    Pages 289-309

  18. Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations

    • Wesley M. Botello-Smith, Yun Lyna Luo
    Pages 311-334

  19. Large-Scale Molecular Dynamics Simulations of Cellular Compartments

    • Eric Wilson, John Vant, Jacob Layton, Ryan Boyd, Hyungro Lee, Matteo Turilli et al.
    Pages 335-356

  20. Back Matter

    Pages 357-358
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Keywords

About this book

This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. 

Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to furtherunderstand these essential proteins and their many biological roles.

Editors and Affiliations

  • School of Biological Sciences and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA

    Ingeborg Schmidt-Krey

  • School of Physics, Georgia Institute of Technology, Atlanta, USA

    James C. Gumbart

Bibliographic Information

  • Book Title: Structure and Function of Membrane Proteins

  • Editors: Ingeborg Schmidt-Krey, James C. Gumbart

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1394-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1393-1Published: 21 April 2021

  • Softcover ISBN: 978-1-0716-1396-2Published: 21 April 2021

  • eBook ISBN: 978-1-0716-1394-8Published: 20 April 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 358

  • Number of Illustrations: 27 b/w illustrations, 70 illustrations in colour

  • Topics: Cell Biology, Membrane Biology, Protein Science

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