Overview
- Editors:
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Boris Hinz
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Laboratory of Tissue Repair and Regeneration, Faculty of Dentistry, University of Toronto, Toronto, Canada
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David Lagares
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Matrix and Mechanobiology Program, Massachusetts General Hospital, Charlestown, USA
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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Table of contents (30 protocols)
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Fundamental Methods to Study Myofibroblast Biology
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- Fereshteh S. Younesi, Dong Ok Son, Joao Firmino, Boris Hinz
Pages 17-47
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- Hesham Soliman, Lin Wei Tung, Fabio M. V. Rossi
Pages 49-84
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- Joanne Porte, Gisli Jenkins, Amanda L. Tatler
Pages 99-108
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- James Griffith, William L. Berry
Pages 109-114
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- Tamara Rosell-GarcÃa, Fernando Rodriguez-Pascual
Pages 115-122
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- Tamara Cruz, Ana L. Mora, Mauricio Rojas
Pages 139-145
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- Simon Stebler, Michael Raghunath
Pages 147-156
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Myofibroblast Mechanobiology
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Front Matter
Pages 157-157
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- Natalie M. Landry, Sunil G. Rattan, Ian M. C. Dixon
Pages 171-179
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- Shuying Yang, Fernando R. Valencia, Benedikt Sabass, Sergey V. Plotnikov
Pages 181-195
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- Michael Kofler, András Kapus
Pages 197-216
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- Alexander J. Whitehead, Natalie J. Kirkland, Adam J. Engler
Pages 217-226
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- Hossam Kadry, David Lagares, Taslim A. Al-Hilal
Pages 227-236
About this book
This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-β activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and extensive, Myofibroblasts: Methods and Protocols is an essential collection for researchers delving into the processes and effects of these important cells.
Editors and Affiliations
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Laboratory of Tissue Repair and Regeneration, Faculty of Dentistry, University of Toronto, Toronto, Canada
Boris Hinz
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Matrix and Mechanobiology Program, Massachusetts General Hospital, Charlestown, USA
David Lagares
