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Host-Fungal Interactions

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Elaine Bignell

    1. MRC Centre for Medical Mycology, University of Exeter, Exeter, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2260)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans

    • Carol A. Woolford, Aaron P. Mitchell
    Pages 1-13

  3. Serological Proteome Analysis for the Characterization of Secreted Fungal Protein Antigens

    • Juliane Macheleidt, Olaf Kniemeyer
    Pages 15-26

  4. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification

    • Quynh T. Phan, Scott G. Filler
    Pages 27-36

  5. CRISPR-Cas9-Mediated Gene Silencing in Cultured Human Epithelia

    • Sara Gago, Nicola L. D. Overton, Paul Bowyer
    Pages 37-47

  6. Analysis of Epithelial Cell Responses to Microbial Pathogens

    • Spyridoula Nikou, Jemima Ho, Olivia Hepworth, Nicole Ponde, Ruth Dickenson, Jonathan P. Richardson et al.
    Pages 49-82

  7. Single-Cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry

    • Margherita Bertuzzi, Gareth J. Howell
    Pages 83-109

  8. Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

    • Jeffrey Scott Fites, Neta Shlezinger, Tobias M. Hohl, Bruce S. Klein
    Pages 111-119

  9. Fungal Bioreporters to Monitor Outcomes of Aspergillus: Host–Cell Interactions

    • Neta Shlezinger, Jeffrey Scott Fites, Bruce S. Klein, Tobias M. Hohl
    Pages 121-132

  10. Candida albicans Interaction with Oral Epithelial Cells: Adhesion , Invasion, and Damage Assays

    • Selene Mogavero, Bernhard Hube
    Pages 133-143

  11. Experimental Evolution of Candida by Serial Passaging in Host Cells

    • Katja Graf, Bernhard Hube, Sascha Brunke
    Pages 145-154

  12. Quantifying Receptor-Mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells

    • Patawee Asamaphan, Gordon D. Brown, Janet A. Willment
    Pages 155-178

  13. Measuring In Vivo Neutrophil Trafficking Responses During Fungal Infection Using Mixed Bone Marrow Chimeras

    • Rebecca A. Drummond, Michail S. Lionakis
    Pages 179-196

  14. Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole

    • Nora Grahl, Caitlin H. Kowalski, Robert A. Cramer
    Pages 197-205

  15. Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections

    • Jorge Amich
    Pages 207-214

  16. A Murine Model for Chronic A. fumigatus Airway Infections

    • Benjamin Ralph, Donald C. Sheppard
    Pages 215-224

  17. Automated Quantitative Analysis of Airway Epithelial Cell Detachment Upon Fungal Challenge

    • Sayema Rahman, Darren D. Thomson, Margherita Bertuzzi
    Pages 225-239

  18. Back Matter

    Pages 241-242
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Keywords

About this book

This volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • MRC Centre for Medical Mycology, University of Exeter, Exeter, UK

    Elaine Bignell

Bibliographic Information

  • Book Title: Host-Fungal Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Elaine Bignell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1182-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1181-4Published: 07 January 2021

  • Softcover ISBN: 978-1-0716-1184-5Published: 07 January 2022

  • eBook ISBN: 978-1-0716-1182-1Published: 06 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 242

  • Number of Illustrations: 11 b/w illustrations, 28 illustrations in colour

  • Topics: Microbiology, Mycology

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