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Lipid Rafts

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Erhard Bieberich

    1. Department of Physiology, University of Kentucky Medical School, Lexington, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2187)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation and Analysis of Lipid Rafts from Neural Cells and Tissues

    • Sara Grassi, Paola Giussani, Laura Mauri, Simona Prioni, Alessandro Prinetti
    Pages 1-25

  3. A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study

    • Chao Qin, Meixia Pan, Xianlin Han
    Pages 27-35

  4. Biochemical Analysis of Lipid Rafts to Study Pathogenic Mechanisms of Neural Diseases

    • Ernesto R. Bongarzone, Maria I. Givogri
    Pages 37-46

  5. Amyloid-β Interactions with Lipid Rafts in Biomimetic Systems: A Review of Laboratory Methods

    • Galya Staneva, Chiho Watanabe, Nicolas Puff, Vesela Yordanova, Michel Seigneuret, Miglena I. Angelova
    Pages 47-86

  6. Extracellular Vesicles Containing Ceramide-Rich Platforms: “Mobile Raft” Isolation and Analysis

    • Ahmed Elsherbini, Haiyan Qin, Zhihui Zhu, Priyanka Tripathi, Guanghu Wang, Simone M. Crivelli et al.
    Pages 87-98

  7. Isolation of Lipid Rafts (Detergent-Resistant Microdomains) and Comparison to Extracellular Vesicles (Exosomes)

    • Glyn Dawson
    Pages 99-112

  8. Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics

    • Mariana Nikolova-Karakashian
    Pages 113-129

  9. Association of Glycolipids and Growth Factor Receptors in Lipid Rafts

    • Jing Wang, Robert K. Yu
    Pages 131-145

  10. Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering

    • Consuelo Gajate, Faustino Mollinedo
    Pages 147-186

  11. Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach

    • Laureano D. Asico, Selim Rozyyev, Annabelle M. Crusan, Pedro A. Jose, Van Anthony M. Villar
    Pages 187-206

  12. Analysis of Lipids in Ceramide-Enriched Membrane Domains

    • Alexander Carpinteiro, Erich Gulbins
    Pages 207-213

  13. Chemotherapeutic Agents-Induced Ceramide-Rich Platforms (CRPs) in Endothelial Cells and Their Modulation

    • Prerna R. Nepali, Adriana Haimovitz-Friedman
    Pages 215-221

  14. Biophysical Analysis of Lipid Domains by Fluorescence Microscopy

    • Ana E. Ventura, Tânia C. B. Santos, Joaquim T. Marquês, Rodrigo F. M. de Almeida, Liana C. Silva
    Pages 223-245

  15. Biophysical Analysis of Lipid Domains in Mammalian and Yeast Membranes by Fluorescence Spectroscopy

    • Carla Sousa, Filipa C. Santos, Andreia Bento-Oliveira, Beatriz Mestre, Liana C. Silva, Rodrigo F. M. de Almeida
    Pages 247-269

  16. Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy

    • Andrew Fuhrer, Amir M. Farnoud
    Pages 271-282

  17. Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components

    • Gyula Batta, Tímea Hajdu, Peter Nagy
    Pages 283-301

  18. Superresolution Microscopy of Sphingolipids

    • Jan Schlegel, Markus Sauer
    Pages 303-311

  19. Detection of Functionalized Sphingolipid Analogs in Detergent-Resistant Membranes of Immune Cells

    • Julian Fink, Jürgen Seibel, Elita Avota
    Pages 313-325

  20. Immunofluorescence Labeling of Lipid-Binding Proteins CERTs to Monitor Lipid Raft Dynamics

    • Caterina Giovagnoni, Simone M. Crivelli, Mario Losen, Pilar Martinez-Martinez
    Pages 327-335
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Keywords

About this book

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning moreabout the function of lipid rafts in many areas of cell biology and medicine. 


Editors and Affiliations

  • Department of Physiology, University of Kentucky Medical School, Lexington, USA

    Erhard Bieberich

Bibliographic Information

  • Book Title: Lipid Rafts

  • Book Subtitle: Methods and Protocols

  • Editors: Erhard Bieberich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0814-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0813-5Published: 09 August 2020

  • Softcover ISBN: 978-1-0716-0816-6Published: 10 August 2021

  • eBook ISBN: 978-1-0716-0814-2Published: 08 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 350

  • Number of Illustrations: 17 b/w illustrations, 62 illustrations in colour

  • Topics: Lipidology, Mass Spectrometry

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