Leukemia Stem Cells

1. Front Matter

   Pages i-xiii

   PDF

2. Introductory Reviews

   1. Front Matter

      Pages 1-1

      PDF

   2. Introduction and Classification of Leukemias

      • Geoffrey Brown

      Pages 3-23

   3. Leukemia Stem Cells: Concept and Implications

      • Isidro Sánchez-García, César Cobaleda

      Pages 25-37

   4. Leukemia Stem Cell Drug Discovery

      • César Cobaleda, Isidro Sánchez-García

      Pages 39-48

3. Protocols

   1. Front Matter

      Pages 49-49

      PDF

   2. Analysis and Isolation of Mouse Leukemic Stem Cells

      • Fang Dong, Haitao Bai, Hideo Ema

      Pages 51-63

   3. Mass Cytometry of Hematopoietic Cells

      • Astraea Jager, Jolanda Sarno, Kara L. Davis

      Pages 65-76

   4. PCR Technology to Identify Minimal Residual Disease

      • Giovanni Cazzaniga, Simona Songia, Andrea Biondi, On Behalf of the EuroMRD Working Group

      Pages 77-94

   5. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies

      • Michaela Kotrova, Nikos Darzentas, Christiane Pott, Monika Brüggemann, On Behalf of the EuroClonality-NGS Working Group

      Pages 95-111

   6. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)

      • Daniel Hein, Arndt Borkhardt, Ute Fischer

      Pages 113-134

   7. Single-Cell Transcriptomic Analysis of Hematopoietic Cells

      • Paulina M. Strzelecka, Anna M. Ranzoni, Ana Cvejic

      Pages 135-158

   8. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

      • Yiran Liang, Thy Truong, Ying Zhu, Ryan T. Kelly

      Pages 159-179

   9. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation

      • Kyomi J. Igarashi, Ryo Yamamoto

      Pages 181-194

   10. Experimental Competitive Bone Marrow Transplant Assays

       • Roxann Hétu-Arbour, Sarah Bouali, Krista M. Heinonen

       Pages 195-214

   11. Human T-ALL Xenografts

       • Patricia Fuentes, María L. Toribio, Sara González-García

       Pages 215-239

   12. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts

       • Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, Pier Giuseppe Pelicci

       Pages 241-258

   13. Leukemic Stem Cell Culture in Cytokine-Free Medium

       • Xiaolei Liu, Peter S. Klein

       Pages 259-265

   14. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid

       • Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, Ronald Hoffman

       Pages 267-280

   15. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors

       • Cristina Prieto, Damia Romero-Moya, Rosa Montes

       Pages 281-298

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.

• Cancer stem cells
• Hematopoietic stem cells
• Tissue culture
• LSCs
• Human leukemias
• Animal models

• Immune System Development and Function Unit, Centro de Biologia Molecular "Severo Ochoa", (CSIC/UAM), Madrid, Spain

  César Cobaleda

• IBMCC, Laboratory 13, CSIC/Universidad de Salamanca, Salamanca, Spain

  Isidro Sánchez-García

Policies and ethics