Overview
- Editors:
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Aileen J. F. King
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Department of Diabetes, School of Life Course Sciences, King’s College London, London, UK
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (21 protocols)
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- Aileen J. F. King, Lydia F. Daniels Gatward, Matilda R. Kennard
Pages 1-10
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- Mototsugu Nagao, Jonathan Lou S. Esguerra, Anna Wendt, Akira Asai, Hitoshi Sugihara, Shinichi Oikawa et al.
Pages 25-54
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- Paraskevi Salpea, Cristina Cosentino, Mariana Igoillo-Esteve
Pages 55-67
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- Sigurd Lenzen, Tanja Arndt, Matthias Elsner, Dirk Wedekind, Anne Jörns
Pages 69-85
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- Dawei Chen, Terri C. Thayer, Li Wen, F. Susan Wong
Pages 87-92
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- Gustaf Christoffersson, Malin Flodström-Tullberg
Pages 93-105
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- James C. Needell, Danny Zipris
Pages 107-114
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- Barbara Ludwig, Eckhard Wolf, Uwe Schönmann, Stefan Ludwig
Pages 115-134
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- Aileen J. F. King, Elisabet Estil·les, Eduard Montanya
Pages 135-147
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- Christian M. Cohrs, Chunguang Chen, Stephan Speier
Pages 149-157
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- Sumeet Pal Singh, Nikolay Ninov
Pages 159-179
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- Lorna I. F. Smith, Thomas G. Hill, James E. Bowe
Pages 181-205
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- Patricia Fonseca Pedro, Anastasia Tsakmaki, Gavin A. Bewick
Pages 207-216
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- Irene Cózar-Castellano, Germán Perdomo
Pages 217-224
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- Aileen J. F. King, Matilda R. Kennard, Manasi Nandi
Pages 225-239
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- Patricio Atanes, Inmaculada Ruz-Maldonado, Oladapo E. Olaniru, Shanta J. Persaud
Pages 241-268
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- Terri C. Thayer, Dimitri Kakabadse, Joanne Boldison, F. Susan Wong
Pages 269-289
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- Irene Cózar-Castellano, Germán Perdomo
Pages 291-300
About this book
This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models. The chapters in this book cover topics such as important considerations when working with mouse models of diabetes, highlighting factors that new investigators may not be aware of and some potential pitfalls in experimental outcomes; main characteristics of some commonly used animal models of diabetes research, ranging from mice to primates; animal models used to study specific aspects of beta-cell biology; and a focus on techniques used to assess blood glucose homeostasis, insulin action, and islet function in vivo and ex vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Animal Models of Diabetes: Methods and Protocols is a valuable resource that will help diabetes researchers design and carry out in vivo studies that will best suit their experimental questions and needs.
Editors and Affiliations
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Department of Diabetes, School of Life Course Sciences, King’s College London, London, UK
Aileen J. F. King