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Optical Spectroscopic and Microscopic Techniques

Analysis of Biological Molecules

  • Highlights recent advances in optical spectroscopic and microscopic techniques used to study biological molecules

  • Discusses techniques for analyzing macromolecular-ligand interactions

  • Summarizes spectroscopic techniques used to analyze macromolecular structure and dynamics

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-vii
  2. Circular Dichroism Spectroscopy: Principle and Application

    • Suchismita Subadini, Pratyush Ranjan Hota, Devi Prasanna Behera, Harekrushna Sahoo
    Pages 19-33
  3. Steady-State Fluorescence Spectroscopy as a Tool to Monitor Protein/Ligand Interactions

    • Roopa Kenoth, Balamurali M. M., Ravi Kanth Kamlekar
    Pages 35-54
  4. Fluorescence Anisotropy: Probing Rotational Dynamics of Biomolecules

    • Gourab Prasad Pattnaik, Hirak Chakraborty
    Pages 55-75
  5. Fluorescence Lifetime: A Multifaceted Tool for Exploring Biological Systems

    • Subhrajit Mohanty, Usharani Subuddhi
    Pages 77-111
  6. From Ensemble FRET to Single-Molecule Imaging: Monitoring Individual Cellular Machinery in Action

    • Farhana Islam, Manali Basu, Padmaja Prasad Mishra
    Pages 113-142
  7. Nanosecond Time-Resolved Fluorescence Assays

    • Yan-Cen Liu, Andreas Hennig
    Pages 143-175
  8. Fluorescence Correlation Spectroscopy: A Highly Sensitive Tool for Probing Intracellular Molecular Dynamics and Disease Diagnosis

    • Bikash Chandra Swain, Anand Kant Das, Janmejaya Rout, Shrutidhara Biswas, Umakanta Tripathy
    Pages 177-195
  9. Principles and Applications of Fluorescence Microscopy

    • Bibhu Ranjan Sarangi
    Pages 197-213
  10. Analysis of Biomolecular Dynamics Under Fourier Transform Infrared Spectroscopy

    • Sanjeev Kumar Paikra, Monalisa Mishra
    Pages 215-241

About this book

This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.

Editors and Affiliations

  • Biophysical and Protein Chemistry Laboratory, Department of Chemistry, National Institute of Technology (NIT) Rourkela, Rourkela, India

    Harekrushna Sahoo

About the editor

Dr. Harekrushna Sahoo is currently working as an Associate Professor of Chemistry at the NIT (National Institute of Technology) in Rourkela, India. He previously served as a guest scientist at the Max-Bergmann Center (Dresden, Germany). He completed his Ph.D. at Jacobs University Bremen (Bremen, Germany) in 2006, prior to engaging in postdoctoral research at the University of Massachusetts (Amherst, USA) and Technical University Dresden (Dresden, Germany). His research chiefly focuses on using biophysical chemistry to understand the kinetics and dynamics of various types of proteins, along with the impacts of environmental stress. He has served as a reviewer for a number of international peer-reviewed and reputed journals, including Journal of Physical Chemistry B, ACS Omega, ACS Sustainable Chemistry and Engineering, Journal of Photochemistry and Photobiology, and International Journal of Biological Macromolecules. He has more than 9 years of teaching experience in General Chemistry, Physical Chemistry, Supramolecular Chemistry, Structure and Function of Biomolecules, Biophysical Chemistry, and Optical Spectroscopy.

He has also published more than 50 research articles in peer-reviewed international journals and authored or co-authored several book chapters. He is a member of many international scientific societies and organizations, e.g. American Chemical Society, Biophysical Society, Indian Photobiology Society, and Orissa Chemical Society.


Bibliographic Information

  • Book Title: Optical Spectroscopic and Microscopic Techniques

  • Book Subtitle: Analysis of Biological Molecules

  • Editors: Harekrushna Sahoo

  • DOI: https://doi.org/10.1007/978-981-16-4550-1

  • Publisher: Springer Singapore

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022

  • Hardcover ISBN: 978-981-16-4549-5Published: 26 February 2022

  • Softcover ISBN: 978-981-16-4552-5Published: 27 February 2023

  • eBook ISBN: 978-981-16-4550-1Published: 25 February 2022

  • Edition Number: 1

  • Number of Pages: VII, 260

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Biological Techniques, Chemistry/Food Science, general, Atomic, Molecular, Optical and Plasma Physics

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access