Overview
- Nominated by Kyoto University as an outstanding Ph.D. thesis
- Includes a review chapter summarizing the history of and recent studies on Py–Im
- Describes in detail the synthesis of Py–Im polyamides using an automatic solid-phase peptide
Part of the book series: Springer Theses (Springer Theses)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (4 chapters)
Keywords
About this book
This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole–imidazole (Py–Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py–Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py–Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py–Im polyamides, particularly the extension of the target sites c
Authors and Affiliations
Bibliographic Information
Book Title: Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres
Authors: Yusuke Kawamoto
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-981-13-6912-4
Publisher: Springer Singapore
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer Nature Singapore Pte Ltd. 2019
Hardcover ISBN: 978-981-13-6911-7Published: 19 March 2019
eBook ISBN: 978-981-13-6912-4Published: 07 March 2019
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XII, 122
Number of Illustrations: 10 b/w illustrations, 52 illustrations in colour