Advances in Experimental Medicine and Biology

Transgenic Ascidians

Editors: Sasakura, Yasunori (Ed.)

  • Summarizes transgenesis techniques in ascidians and up-to-date concrete research using those techniques
  • Enables readers to quickly access and compare techniques at a glance
  • Helps readers to apply the strategies contained here to their own studies
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eBook 130,89 €
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  • ISBN 978-981-10-7545-2
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Hardcover 161,19 €
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  • ISBN 978-981-10-7544-5
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About this book

This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies.

Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates.

This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.

About the authors

Yasunori Sasakura started his research career in Dr. Noriyuki Satoh’s laboratory in 1996. His Ph.D. thesis was about the localization of mRNAs in eggs of the ascidian Halocynthia roretzi. He then focused on developing the genetic approaches in the ascidian Ciona intestinalis. The reason is that he thought that genetics would be necessary to elucidate molecular mechanisms of maternally stored substances. He introduced transposon-mediated germ cell transgenesis and mutagenesis in this ascidian. From then on he brought many technical innovations in the study of Ciona with transgenic techniques. For example, his group has introduced transgenesis-mediated tissue/cell tracing with the photoconvertible protein Kaede, transposon-based specific knockdown of maternally transcribed RNAs, the optogenetic approach to understand neuronal functions, and genome editing techniques in Ciona with TALENs and CRISPR/Cas9.

Table of contents (16 chapters)

  • Introduction

    Sasakura, Yasunori

    Pages 1-3

  • Microinjection of Exogenous Nucleic Acids into Eggs: Ciona Species

    Kobayashi, Kenji (et al.)

    Pages 5-13

  • Practical Guide for Ascidian Microinjection: Phallusia mammillata

    Yasuo, Hitoyoshi (et al.)

    Pages 15-24

  • Microinjection of Exogenous DNA into Eggs of Halocynthia roretzi

    Kumano, Gaku

    Pages 25-35

  • Electroporation in Ascidians: History, Theory and Protocols

    Zeller, Robert W.

    Pages 37-48

Buy this book

eBook 130,89 €
price for Spain (gross)
  • ISBN 978-981-10-7545-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 161,19 €
price for Spain (gross)
  • ISBN 978-981-10-7544-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Transgenic Ascidians
Editors
  • Yasunori Sasakura
Series Title
Advances in Experimental Medicine and Biology
Series Volume
1029
Copyright
2018
Publisher
Springer Singapore
Copyright Holder
Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-10-7545-2
DOI
10.1007/978-981-10-7545-2
Hardcover ISBN
978-981-10-7544-5
Series ISSN
0065-2598
Edition Number
1
Number of Pages
X, 201
Topics