Advances in Experimental Medicine and Biology

Biological Small Angle Scattering: Techniques, Strategies and Tips

Editors: Chaudhuri, B., Muñoz, I.G., Qian, S., Urban, V.S. (Eds.)

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  • Includes the principles and theoretical background of various SAS techniquesStates practical aspects that range from sample preparation to data publication
  • Covers techniques for improving data quality and analysis, as well as different scientific applications of SAS.
  • Gives abundant illustrations and practical tips
  • Explains clearly the principles and the reviews on the latest progresses and serves as a guide through all aspects of biological solution SAS for readers
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eBook 128,39 €
price for Spain (gross)
  • ISBN 978-981-10-6038-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-981-10-6037-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 166,39 €
price for Spain (gross)
  • ISBN 978-981-13-5555-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications.

The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS.

The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications.

Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Small Angle Scattering: Historical Perspective and Future Outlook

    Pages 1-10

    Weiss, Thomas M.

  • Sample and Buffer Preparation for SAXS

    Pages 11-30

    Graewert, Melissa A. (et al.)

  • Considerations for Sample Preparation Using Size-Exclusion Chromatography for Home and Synchrotron Sources

    Pages 31-45

    Rambo, Robert P.

  • How to Analyze and Present SAS Data for Publication

    Pages 47-64

    Brennich, Martha (et al.)

  • Designing and Performing Biological Solution Small-Angle Neutron Scattering Contrast Variation Experiments on Multi-component Assemblies

    Pages 65-85

    Krueger, Susan

Buy this book

eBook 128,39 €
price for Spain (gross)
  • ISBN 978-981-10-6038-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-981-10-6037-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 166,39 €
price for Spain (gross)
  • ISBN 978-981-13-5555-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Biological Small Angle Scattering: Techniques, Strategies and Tips
Editors
  • Barnali Chaudhuri
  • Inés G. Muñoz
  • Shuo Qian
  • Volker S. Urban
Series Title
Advances in Experimental Medicine and Biology
Series Volume
1009
Copyright
2017
Publisher
Springer Singapore
Copyright Holder
Springer Nature Singapore Pte Ltd.
eBook ISBN
978-981-10-6038-0
DOI
10.1007/978-981-10-6038-0
Hardcover ISBN
978-981-10-6037-3
Softcover ISBN
978-981-13-5555-4
Series ISSN
0065-2598
Edition Number
1
Number of Pages
X, 268
Number of Illustrations
10 b/w illustrations, 77 illustrations in colour
Topics