Plant Tissue Culture Manual - Supplement 7

1. Front Matter

   Pages i-xiii

   PDF

2. Basic Techniques — Cell & Tissue Culture of Model Species

   1. Front Matter

      Pages xv-xv

      PDF

   2. Media preparation

      • Oluf L. Gamborg

      Pages 1-24

   3. The initiation and maintenance of callus cultures of carrot and tobacco

      • Robert D. Hall

      Pages 25-43

   4. The initiation and maintenance of plant cell suspension cultures

      • Robert D. Hall

      Pages 45-65

   5. Shoot cultures and root cultures of tobacco

      • Jennifer F. Topping, Keith Lindsey

      Pages 67-79

   6. Somatic embryogenesis in orchardgrass

      • Michael E. Horn

      Pages 81-95

   7. Arabidopsis Regeneration and Transformation (Leaf & Cotyledon Explant System)

      • Renate Schmidt, Lothar Willmitzer

      Pages 97-113

   8. Arabidopsis protoplast transformation and regeneration

      • Brigitte Damm, Lothar Willmitzer

      Pages 115-134

   9. Arabidopsis regeneration and transformation (Root Explant System)

      • Dirk Valvekens, Mieke van Lijsebettens, Marc van Montagu

      Pages 135-151

   10. Somatic Embryogenesis in Carrot (Daucus carota)

       • Abraham D. Krikorian, David L. Smith

       Pages 153-184

   11. Low density cultures: microdroplets and single cell nurse cultures

       • G. Spangenberg, H.-U. Koop

       Pages 185-212

   12. Tobacco Protoplast Isolation, Culture and Regeneration

       • I. Negrutiu

       Pages 213-223

3. Tissue Culture & Transformation of Crop Species

   1. Front Matter

      Pages 225-225

      PDF

   2. Embryogenic callus, cell suspension and protoplast cultures of cereals

      • Indra K. Vasil, Vimla Vasil

      Pages 227-242

   3. Transformation and regeneration of rice protoplasts

      • Junko Kyozuka, Ko Shimamoto

      Pages 243-259

   4. Transformation and regeneration of orchardgrass protoplasts

      • Michael E. Horn

      Pages 261-275

   5. Transformation and regeneration of oilseed rape protoplasts

      • Dominique Rouan, Philippe Guerche

      Pages 277-300

   6. Regeneration and transformation of potato by Agrobacterium tumefaciens

      • Richard G. F. Visser

      Pages 301-309

   7. Transformation of tomato with Agrobacterium tumefaciens

      • Sheila McCormick

      Pages 311-319

Plant tissue culture has a long history, dating back to the work of Gottlieb Haberlandt and others at the end of the 19th century, but the associated concepts and techniques have reached a level of usefulness and application which has never been greater. The technical innovations have given new insights into fundamental aspects of plant differentiation and development, and have paved the way to the identification of strategies for the genetic manipula­ tion of plants. It is the aim of this manual to deliver a broad range of these techniques in a form which is accessible to students and research scientists of diverse backgrounds, including those with little or no previous experience. The themes of the manual aim to reflect those research areas which have been advanced by tissue culture technology. As was the case for the sister volume Plant Molecular Biology Manual, the objective has been from the start to produce a manual which is at home on the laboratory bench. The plastic-covered, ring-bound format has proved to be most popular and is retained here. Equally, the emphasis has been on producing a collection of detailed step-by-step protocols, each supplemented with an introductory text and practical footnotes, to provide the next best thing to a supervisor at one's shoulder.

• conservation
• gene transfer
• genes
• genetic engineering
• molecular biology
• mutant
• physiology

• Department of Biological Sciences, University of Durham, Leicester, UK

  K. Lindsey

Policies and ethics