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Springer Protocols Handbooks Hydrocarbon and Lipid Microbiology Protocols

Hydrocarbon and Lipid Microbiology Protocols

Bioproducts, Biofuels, Biocatalysts and Facilitating Tools

Editors: McGenity, Terry J., Timmis, Kenneth N., Fernandez, Balbina Nogales (Eds.)

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  • Offers readily reproducible, step-by-step laboratory methods
  • Provides helpful tips and tricks to complement the protocols
  • Includes troubleshooting advice
  • Features practical laboratory guidelines to promote successful results
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eBook 79,72 €
price for Spain (gross)
  • ISBN 978-3-662-53115-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 135,19 €
price for Spain (gross)
  • ISBN 978-3-662-53113-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 98,79 €
price for Spain (gross)
  • ISBN 978-3-662-57114-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Table of contents (14 chapters)

Table of contents (14 chapters)
  • Introduction to Bioproducts, Biofuels, Biocatalysts and Facilitating Tools

    Pages 1-2

    Verstraete, Willy

  • Genetic Enzyme Screening System: A Method for High-Throughput Functional Screening of Novel Enzymes from Metagenomic Libraries

    Pages 3-12

    Kim, Haseong (et al.)

  • Functional Screening of Metagenomic Libraries: Enzymes Acting on Greasy Molecules as Study Case

    Pages 13-36

    Martínez-Martínez, Mónica (et al.)

  • Screening for Enantioselective Lipases

    Pages 37-69

    Classen, Thomas (et al.)

  • Use of Bacterial Polyhydroxyalkanoates in Protein Display Technologies

    Pages 71-86

    Hay, Iain D. (et al.)

Buy this book

eBook 79,72 €
price for Spain (gross)
  • ISBN 978-3-662-53115-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 135,19 €
price for Spain (gross)
  • ISBN 978-3-662-53113-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 98,79 €
price for Spain (gross)
  • ISBN 978-3-662-57114-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle
Bioproducts, Biofuels, Biocatalysts and Facilitating Tools
Editors
  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales Fernandez
Series Title
Springer Protocols Handbooks
Copyright
2017
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-53115-0
DOI
10.1007/978-3-662-53115-0
Hardcover ISBN
978-3-662-53113-6
Softcover ISBN
978-3-662-57114-9
Series ISSN
1949-2448
Edition Number
1
Number of Pages
X, 245
Number of Illustrations
20 b/w illustrations, 30 illustrations in colour
Topics