Overview
- Offers readily reproducible, step-by-step laboratory methods
- Provides helpful tips and tricks to complement the protocols
- Includes troubleshooting advice
- Features practical laboratory guidelines to promote successful results
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (12 protocols)
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Front Matter
Keywords
About this book
This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Editors and Affiliations
Bibliographic Information
Book Title: Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle: Microbial Quantitation, Community Profiling and Array Approaches
Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-3-662-52778-8
Publisher: Springer Berlin, Heidelberg
eBook Packages: Springer Protocols
Copyright Information: Springer-Verlag Berlin Heidelberg 2017
Hardcover ISBN: 978-3-662-52776-4Published: 29 December 2016
Softcover ISBN: 978-3-662-57081-4Published: 07 July 2018
eBook ISBN: 978-3-662-52778-8Published: 19 December 2016
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: X, 252
Number of Illustrations: 18 b/w illustrations, 12 illustrations in colour
Topics: Microbiology, Biochemical Engineering