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Springer Protocols Handbooks Hydrocarbon and Lipid Microbiology Protocols

Hydrocarbon and Lipid Microbiology Protocols

Microbial Quantitation, Community Profiling and Array Approaches

Editors: McGenity, Terry J., Timmis, Kenneth N., Fernandez, Balbina Nogales (Eds.)

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  • Offers readily reproducible, step-by-step laboratory methods
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eBook 79,72 €
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  • ISBN 978-3-662-52778-8
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Hardcover 135,19 €
price for Spain (gross)
  • ISBN 978-3-662-52776-4
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  • Usually dispatched within 3 to 5 business days.
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Softcover 98,79 €
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  • ISBN 978-3-662-57081-4
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  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Table of contents (12 chapters)

Table of contents (12 chapters)
  • Introduction to Microbial Quantitation, Community Profiling, and Array Approaches

    Pages 1-5

    Neufeld, Josh D.

  • A Cell Extraction Method for Oily Sediments

    Pages 7-16

    Lappé, Michael (et al.)

  • Introduction to Microplate MPN Enumeration of Hydrocarbon Degraders

    Pages 17-33

    Johnsen, Anders R.

  • Primers for dsr Genes and Most Probable Number Method for Detection of Sulfate-Reducing Bacteria in Oil Reservoirs

    Pages 35-43

    Shen, Yin (et al.)

  • Real-Time PCR Approaches for Analysis of Hydrocarbon-Degrading Bacterial Communities

    Pages 45-64

    McKew, Boyd A. (et al.)

Buy this book

eBook 79,72 €
price for Spain (gross)
  • ISBN 978-3-662-52778-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 135,19 €
price for Spain (gross)
  • ISBN 978-3-662-52776-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 98,79 €
price for Spain (gross)
  • ISBN 978-3-662-57081-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle
Microbial Quantitation, Community Profiling and Array Approaches
Editors
  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales Fernandez
Series Title
Springer Protocols Handbooks
Copyright
2017
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-52778-8
DOI
10.1007/978-3-662-52778-8
Hardcover ISBN
978-3-662-52776-4
Softcover ISBN
978-3-662-57081-4
Series ISSN
1949-2448
Edition Number
1
Number of Pages
X, 252
Number of Illustrations
18 b/w illustrations, 12 illustrations in colour
Topics