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Springer Protocols Handbooks Hydrocarbon and Lipid Microbiology Protocols

Hydrocarbon and Lipid Microbiology Protocols

Genetic, Genomic and System Analyses of Communities

Editors: McGenity, Terry J., Timmis, Kenneth N., Fernandez, Balbina Nogales (Eds.)

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eBook 118,99 €
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  • ISBN 978-3-662-50450-5
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Hardcover 145,59 €
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  • ISBN 978-3-662-50449-9
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  • Usually dispatched within 3 to 5 business days.
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Softcover 145,59 €
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  • ISBN 978-3-662-57062-3
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  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities.
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Table of contents (14 chapters)

Table of contents (14 chapters)
  • Introduction to Genetic, Genomic, and System Analyses for Communities

    Pages 1-4

    Gilbert, Jack A. (et al.)

  • Genomic Analysis of Pure Cultures and Communities

    Pages 5-27

    Toshchakov, Stepan V. (et al.)

  • Protocols for Metagenomic Library Generation and Analysis in Petroleum Hydrocarbon Microbe Systems

    Pages 29-49

    Moormann, Stephanie M. (et al.)

  • Preparation and Analysis of Metatranscriptomic Libraries in Petroleum Hydrocarbon Microbe Systems

    Pages 51-67

    Hampton-Marcell, Jarrad T. (et al.)

  • Prokaryotic Metatranscriptomics

    Pages 69-98

    Pérez-Pantoja, Danilo (et al.)

Buy this book

eBook 118,99 €
price for Spain (gross)
  • ISBN 978-3-662-50450-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 145,59 €
price for Spain (gross)
  • ISBN 978-3-662-50449-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 145,59 €
price for Spain (gross)
  • ISBN 978-3-662-57062-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Hydrocarbon and Lipid Microbiology Protocols
Book Subtitle
Genetic, Genomic and System Analyses of Communities
Editors
  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales Fernandez
Series Title
Springer Protocols Handbooks
Copyright
2017
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-50450-5
DOI
10.1007/978-3-662-50450-5
Hardcover ISBN
978-3-662-50449-9
Softcover ISBN
978-3-662-57062-3
Series ISSN
1949-2448
Edition Number
1
Number of Pages
X, 288
Number of Illustrations
20 b/w illustrations, 30 illustrations in colour
Topics