Hydrocarbon and Lipid Microbiology Protocols
Primers
Editors: McGenity, Terry J., Timmis, Kenneth N., Fernandez, Balbina Nogales (Eds.)
Free Preview- Offers readily-reproducible step-by-step laboratory methods
- Provides helpful tips and tricks supporting the protocols
- Includes troubleshooting advice
- Contains practical laboratory guidance to promote successful results
Buy this book
- About this book
-
This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle.
Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
- Table of contents (9 chapters)
-
-
Introduction to Primer-Based Detection of Microbial Genes, Particularly Those Encoding Enzymes for Aromatic/Aliphatic Hydrocarbon Biodegradation
Pages 1-7
-
Current Landscape of Biomolecular Approaches for Assessing Biodegradation of Aromatic Hydrocarbons
Pages 9-21
-
Primers: Bacterial Genes Encoding Enzymes for Aerobic Hydrocarbon Degradation
Pages 23-37
-
Primers: Functional Genes for Anaerobic Hydrocarbon Degrading Microbes
Pages 39-55
-
Primers: Functional Marker Genes for Methylotrophs and Methanotrophs
Pages 57-77
-
Table of contents (9 chapters)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Hydrocarbon and Lipid Microbiology Protocols
- Book Subtitle
- Primers
- Editors
-
- Terry J. McGenity
- Kenneth N. Timmis
- Balbina Nogales Fernandez
- Series Title
- Springer Protocols Handbooks
- Copyright
- 2017
- Publisher
- Springer-Verlag Berlin Heidelberg
- Copyright Holder
- Springer-Verlag Berlin Heidelberg
- eBook ISBN
- 978-3-662-50428-4
- DOI
- 10.1007/978-3-662-50428-4
- Hardcover ISBN
- 978-3-662-50427-7
- Softcover ISBN
- 978-3-662-57057-9
- Series ISSN
- 1949-2448
- Edition Number
- 1
- Number of Pages
- X, 241
- Number of Illustrations
- 10 illustrations in colour
- Topics
