Functional Neuroanatomy

1. Front Matter

   Pages I-XVI

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2. Electron Microscopy of Golgi-Impregnated Neurons

   • Willi A. Ribi

   Pages 1-18

3. Block Intensification and X-Ray Microanalysis of Cobalt-Filled Neurons for Electron Microscopy

   • Ursula K. Bassemir, Nicholas J. Strausfeld

   Pages 19-43

4. Horseradish Peroxidase and Other Heme Proteins as Neuronal Markers

   • Dick R. Nässel

   Pages 44-91

5. Intracellular Staining with Nickel Chloride

   • Fred Delcomyn

   Pages 92-95

6. Rubeanic Acid and X-Ray Microanalysis for Demonstrating Metal Ions in Filled Neurons

   • Carole M. Hackney, Jennifer S. Altman

   Pages 96-111

7. Double Marking for Light and Electron Microscopy

   • Harjit Singh Seyan, Ursula K. Bassemir, Nicholas J. Strausfeld

   Pages 112-131

8. Lucifer Yellow Histology

   • Nicholas J. Strausfeld, Harjit Singh Seyan, David Wohlers, Jonathan P. Bacon

   Pages 132-155

9. Portraying the Third Dimension in Neuroanatomy

   • Peter T. Speck, Nicholas J. Strausfeld

   Pages 156-182

10. Three-Dimensional Reconstruction and Stereoscopic Display of Neurons in the Fly Visual System

    • Roland Hengstenberg, Heinrich Bülthoff, Bärbel Hengstenberg

    Pages 183-205

11. Laser Microsurgery for the Study of Behaviour and Neural Development of Flies

    • Gad Geiger, Dick R. Nässel, Harjit Singh Seyan

    Pages 206-224

12. Anatomical Localization of Functional Activity in Flies Using ³H-2-Deoxy-d-Glucose

    • Erich Buchner, Sigrid Buchner

    Pages 225-238

13. Strategies for the Identification of Amine- and Peptide-Containing Neurons

    • Michael E. Adams, Cynthia A. Bishop, Michael O’Shea

    Pages 239-249

14. Immunochemical Identification of Vertebrate-Type Brain-Gut Peptides in Insect Nerve Cells

    • Hanne Duve, Alan Thorpe

    Pages 250-266

15. Immunocytochemical Techniques for the Identification of Peptidergic Neurons

    • Manfred Eckert, Joachim Ude

    Pages 267-301

16. Detection of Serotonin-Containing Neurons in the Insect Nervous System by Antibodies to 5-HT

    • Nikolai Klemm

    Pages 302-316

17. Monoaminergic Innervation in a Hemipteran Nervous System: A Whole-Mount Histofluorescence Survey

    • Thomas R. J. Flanagan

    Pages 317-330

18. Identification of Neurons Containing Vertebrate-Type Brain-Gut Peptides by Antibody and Cobalt Labelling

    • Hanne Duve, Alan Thorpe, Nicholas J. Strausfeld

    Pages 331-338

19. Interpretation of Freeze-Fracture Replicas of Insect Nervous Tissue

    • Stanley D. Carlson, Richard L. Saint Marie, Che Chi

    Pages 339-375

20. High-Voltage Electron Microscopy for Insect Neuroanatomy

    • Che Chi

    Pages 376-385

The "functional" in the title of this book not only reflects my personal bias about neuroanatomy in brain research, it is also the gist of many chapters which describe sophisticated ways to resolve structures and interpret them as dynamic entities. Examples are: the visualization of functionally identified brain areas or neurons by activity staining or intracellular dye-iontophoresis; the resolution of synaptic connections between physiologically identified nerve cells; and the biochemical identification of specific neurons (their peptides and transmitters) by histo- and immunocytochemistry. I personally view the nervous system as an organ whose parts, continuously exchanging messages, arrive at their decisions by the cooperative phenome­ non of consensus and debate. This view is, admittedly, based on my own ex­ perience of looking at myriads of nerve cells and their connections rather than studying animal behaviour or theorizing. Numerous structural studies have demonstrated that interneurons in the brain must receive hundreds of thousands of synapses. Many neurons receive inputs from several different sensory areas: each input conveys a message about the external world and possibly also about past events which are stored within the central nervous system. Whether an interneuron responds to a certain combination of inputs may be, literally, a matter of debate whose outcome is decided at the post­ synaptic membrane. A nerve cell responding to an overriding command is possibly a rare event.

• Nervous System
• Neuroanatomy
• anatomy
• neurons
• visualization

• European Molecular Biology Laboratory, Heidelberg, Germany

  Nicholas J. Strausfeld

Policies and ethics