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Springer Series in Biophysics

Electron Probe Microanalysis

Applications in Biology and Medicine

Editors: Zierold, Karl, Hagler, Herbert K. (Eds.)

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  • ISBN 978-3-642-74477-8
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About this book

The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti­ cular diffusible ions (elements). 2. Electron probe microanaly­ sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con­ verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo­ gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • The History of Electron Probe Microanalysis in Biology

    Pages 1-15

    Hall, T. A.

  • Specimen Preparation and Other Limitations in Quantitative Eletron Probe X-Ray Microanalysis (EPXMA) Using Ultrathin Sections

    Pages 17-32

    Roos, Norbert

  • Freeze-Substitution and Low Temperature Embedding for Analytical Electron Microscopy

    Pages 33-46

    Edelmann, L.

  • Ensuring the Validity of Results in Biological X-Ray Microanalysis

    Pages 47-58

    Zglinicki, Thomas

  • The Subcellular Accumulation of Toxic Heavy Metals: Qualitative and Quantitative X-Ray Microanalysis

    Pages 59-72

    Morgan, A. J. (et al.)

Buy this book

eBook 106,99 €
price for Spain (gross)
  • ISBN 978-3-642-74477-8
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover 135,19 €
price for Spain (gross)
  • ISBN 978-3-642-74479-2
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Electron Probe Microanalysis
Book Subtitle
Applications in Biology and Medicine
Editors
  • Karl Zierold
  • Herbert K. Hagler
Series Title
Springer Series in Biophysics
Series Volume
4
Copyright
1989
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-74477-8
DOI
10.1007/978-3-642-74477-8
Softcover ISBN
978-3-642-74479-2
Series ISSN
0932-2353
Edition Number
1
Number of Pages
XVI, 322
Topics