Microarrays in Diagnostics and Biomarker Development

1. Front Matter

   Pages i-viii

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2. The Microarray Paradigm and Its Various Implementations

   • Bertrand Jordan

   Pages 1-8

3. DNA Arrays

   1. Front Matter

      Pages 9-9

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   2. DNA Arrays in Many Guises

      • Bertrand Jordan

      Pages 11-19

   3. Expression Profiling for Diagnostics

      • Bertrand Jordan

      Pages 21-27

   4. Genotype Characterisation for Diagnostics

      • Bertrand Jordan

      Pages 29-38

   5. Array-CGH and SNP-Arrays, the New Karyotype

      • Karoly Szuhai

      Pages 39-52

4. Protein (Antigen and Antibody) and Peptide Arrays

   1. Front Matter

      Pages 53-53

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5. Protein (Antigen & Antibody) and Peptide Arrays

   1. Protein Microarrays and Their Potential Clinical Applications in the Era of Personalized Medicine

      • Hong Zhang, Steven Pelech

      Pages 55-80

   2. Peptide Arrays

      • Hong Zhang, Steven Pelech, Rob Ruijtenbeek, Thomas Felgenhauer, Ralf Bischoff, Frank Breitling et al.

      Pages 81-112

   3. Slow Off-Rate Modified Aptamer Arrays for Biomarker Discovery and Diagnostic Applications

      • Jeffrey J. Walker, Edward N. Brody, Larry Gold

      Pages 113-131

6. Other Types of Arrays

   1. Front Matter

      Pages 133-133

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7. Other types of arrays

   1. Tissue Microarrays for Translational Research

      • Ronald Simon, Martina Mirlacher

      Pages 135-152

   2. Recent and Future Developments of Microarrays: Miniaturization and Lab-on-Chip Approaches

      • Inbal Tsarfati-BarAd, Levi A. Gheber

      Pages 153-168

8. Data Analysis, Commercial and Regulatory Aspects

   1. Front Matter

      Pages 169-169

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9. Data analysis, commercial and regulatory aspects

   1. Analysis of Array Data and Clinical Validation of Array-Based Assays

      • Benjamin Haibe-Kains, John Quackenbush

      Pages 171-210

   2. Challenges in the Commercialization of Array-Based Tests

      • Justin Petrone

      Pages 211-227

   3. Genes, Microarrays, and Patents

      • Justin Petrone

      Pages 229-236

10. Back Matter

    Pages 237-240

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Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards “nanoarrays”, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.

• Aptamer
• Expression Profiling
• Genotype Characterization
• Lab-on-Chip Approach
• Peptide Arrays
• Protein Arrays
• microarrays

• CNRS, La Cadière d’Azur, France

  Bertrand Jordan

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