Antibody Engineering Volume 1
Editors: Kontermann, Roland E., Dübel, Stefan (Eds.)
Free Preview- The updated second edition of the successful Springer Lab Manual “Antibody Engineering” is now offered in two volumes with nearly the double number of protocols
- With both new and well established techniques
- Detailed step-by-step protocols including all materials and practical advices
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- About this book
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Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity.
Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.
- Table of contents (50 chapters)
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Cloning of Variable Domains from Mouse Hybridoma by PCR
Pages 3-14
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Coning Hybridoma cDNA by RACE
Pages 15-20
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Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly
Pages 21-44
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Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens
Pages 47-63
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Human Antibody Gene Libraries
Pages 65-84
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Table of contents (50 chapters)
- Download Preface 1 PDF (25.9 KB)
- Download Sample pages 1 PDF (59.5 KB)
- Download Table of contents PDF (32.4 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Antibody Engineering Volume 1
- Editors
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- Roland E. Kontermann
- Stefan Dübel
- Series Title
- Springer Protocols Handbooks
- Copyright
- 2010
- Publisher
- Springer-Verlag Berlin Heidelberg
- Copyright Holder
- Springer-Verlag GmbH Germany, part of Springer Nature
- eBook ISBN
- 978-3-642-01144-3
- DOI
- 10.1007/978-3-642-01144-3
- Hardcover ISBN
- 978-3-642-01143-6
- Softcover ISBN
- 978-3-662-49594-0
- Series ISSN
- 1949-2448
- Edition Number
- 2
- Number of Pages
- XIII, 788
- Additional Information
- Originally published in one volume, within the series "Springer Lab Manuals"
- Topics
